The RBS-controlled translation strength of PdxST expression was optimized by the RBS calculator using moderate and weak strength (Supplementary Table 1). We obtained two engineered strains (named LL003 and LL005). When all the strains were cultured in an LB medium (as shown in Fig. 2b), ...
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest. Volume (to add to vial) ...
Reconstitution Calculator The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.
We performed evolution with the ESM-1b language model and the ESM-1v ensemble of five language models (six language models in total)19,20. ESM-1b and ESM-1v were trained on UniRef50 and UniRef90, respectively, which are protein sequence datasets that represent variation across millions of ...
Protein-protein interaction is important in molecular and cellular biology for catalysis, regulation, immune response, and inhibition. Therefore, it is of interest to decipher PPI for the understanding of molecular biology events. Experimental methods su
The MachineLearning Impact calculator [86] is used to estimate the carbon footprint.7 The estimates were made on the basis of equivalent calculations on the Google Cloud Platform ’europe-north1’ with 0.21 kg CO2 emission per kWh and the comparison can be seen in Table 5. Table 5. ...
was observed in Symbiodiniaceae. Meanwhile, the low values of protein sequence similarity are potentially linked to parasitism, as this way of life often coincides with relaxed functional constraints leading to higher substitution rates [65]. The non-canonical splicing sites, the large diversity of...
Recombination rates for allD. melanogasterorphans was calculated using the Recombination Rate Calculator (RRC) (Fiston-Lavier et al.2010), utilising the experimentally determined crossover rates published by Comeron et al. (2012). Gene start and end coordinates were taken for eachD. melanogasteror...
[50] is used to calculate peptide related parameters: instability index [27], molecular weight and theoretical pI. A dynamic charge calculator implemented using Biophp [34] is used for sequence feature calculation has been incorporated in the server. The PeptideMine server runs on an Apache web...
Reconstitution Calculator The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.