蛋白质-蛋白质相互作用(protein-protein interaction,PPI)是指两个或两个以上的蛋白质分子通过非共价键形成蛋白质复合体(protein complex)的过程。研究蛋白之间的相互作用网络,有助于挖掘核心的调控基因,目前已经有很多的蛋白质相互作用的数据库,在这里,...
预测值可用于识别实验得到的ppi中的高置信度以及潜在的假阴性子集。加速interactome mapping以及完善实验未得到的ppi gap。 根据数据类型,ppi预测方法分类为:genomic data, protein sequence, protein structure, PPI networks, gene expression, and annotations of gene function, localization, and process。基于genomic ...
Synonyms Protein interaction network ; Protein physical interaction network Definition A protein-protein interaction (PPI) network is a network or graph representation of physical interactions between protein pairs in an organism. In the network, each node/vertex represents a protein and each edge/link...
There are a multitude of methods that have been proposed to detect protein-protein interactions and populate protein-protein interaction networks. In general, none of them is better than the others but each method has its own strengths and weaknesses. The goodness of a method is measured in ter...
Protein-protein interaction (PPI) networks are commonly-used infrastructure to infer signaling pathways. However, PPI networks generally carry no information of upstream/downstream relationship between interacting proteins, which retards our inferring the signal flow of signaling pathways. In this work, we...
Chakraborty C, Doss C GP, Chen L and Zhu H: Evaluating protein-protein interaction (PPI) networks for diseases pathway, target discovery, and drug-design using `in silico pharmacology'. Curr Protein Pept Sci 15: 561-571, 2014.Chakraborty C, Doss CG, Chen L, Zhu H (2014) Evaluating ...
蛋白质与蛋白质相互作用（Protein-Protein Interaction, PPI）研究方法概述目前检验蛋白质之间相互作用的实验方法主要有：酵母双杂交系统（Yeast two-hybrid system）、噬菌体展示技术（Phage display）、串联亲和纯化-质谱（Tandem affinity purification-mass spectrometry, TAP-MS）以及GST pull-down。其原理与适用范围如下...
Structural insight of the protein–protein interaction (PPI) interface can provide knowledge about the kinetics, thermodynamics and molecular functions of the complex while elucidating its role in diseases and further enabling it as a potential therapeut
Defining genetic interaction GENE INTERACTIONS STRINGdatabase的挖掘 这个数据库绝对是做实验人的宝藏,里面包含了各种蛋白互作关系,不用做实验就有一大堆证据。 IPA了解一下,收费的高端分析软件,大部分就是整合的这个数据库,很多大佬喜欢用IPA来找明星基因,再来讲故事,实例请看之前解读的CSC paper。
揭示PPI网络中的社团结构,对于理解其复杂相互作用的结构和动态特征,了解活体细胞的结构和功能都有很大作用。 Detecting community structure in protein-protein interaction network can help to understand its ...