使用深度几何表示建模突变对蛋白质结合亲和力的影响-Deep geometric representations for modeling effects of mutations on protein-protein binding affinity文章地址: Deep geometric representations for mod…
Protein-binding affinity chromatography mediaAffinity chromatography media are prepared by reacting mono or di-chloro triazine or related dyes with a non-cellulosic solid support matrix containing free hydroxy or amine groups in the presence of an alkali metal hydroxide. In the absence of cyanogen ...
Lina Dong,Yulin Li,Dandan Liu,Ye Ji,Bo Hu,Shuai Shi,Fangyan Zhang,Junjie Hu,Kun Qian,Xianmin Jin,Binju Wang, Prediction of Protein-Ligand Binding Affinity by a Hybrid Quantum-Classical Deep Learning Algorithm,ADVANCED QUANTUM TECHNOLOGIES,2023,https://doi.org/10.1002/qute.202300107 3区...
The fusion of a self-cleaving intein sequence followed by a chitin-binding domain (CBD) allows for one-step chromatographic purification of an untagged protein through the thiol-catalyzed cleavage of the intein sequence from the desired protein. The affinity purification is highly specific and can ...
We presented a novel machine learning method for protein affinity prediction that uses both protein structure and sequence information during training but needs only sequence information for testing. To the best of our knowledge, this is first attempt to combine protein structure and sequence information...
W. et al. JadX is a disparate natural product binding protein. J. Am. Chem. Soc. 138, 2200–2208 (2016). 19. Forget, S. M. et al. Kinetic evaluation of glucose 1-phosphate analogues with a thymidylyltransferase using a continuous coupled enzyme assay. Org. Biomol. Chem. 13, 866...
In our work, a residue in the DNA-binding protein is defined as a binding site if the distance between any protein atoms and DNA atoms is ≤5.0. Regression models and performance evaluation We train the stacking heterogeneous ensemble method using the selected features for every class of ...
Immunoglobulin-binding protein-based affinity chromatography in bispecific antibody purification: Functions beyond product capture Protein Expression and Purification Journal2021, Protein Expression and Purification Yifeng Li 3 Protein L Protein L is a bacterial surface protein that binds to the variable region...
including protein-protein, protein-small molecule, and protein-nucleic acid interactions. Its sensitivity and speed make it a valuable tool for characterizing binding kinetics, affinity constants, and concentration-dependent effects, providing crucial insights into the mechanisms underlying biological processes...
the slope is related to the equilibrium-binding constant of the compound and protein,K, and the intercept being equal to the concentration of the binding protein. For a situation where binding to a number of different sites on protein, or sites on a multitude of proteins, occurs over the co...