Technically, fat westerns are based on immunoblotting techniques in which lipid-protein binding precedes the antibody detection steps. Creative BioMart also provides assistance in experimental design, training to conduct assays and data interpretation in protein-lipids/nucleic acid interaction assay and ...
With any one membrane protein, the tightly coupled lipid might be specific; that is, the interaction might require that the phospholipid contain specific fatty acid chains or particular polar head groups. There is at present, however, no satisfactory direct evidence for such a distinctive lipid ...
This chapter provides a systematic guide for conducting protein–lipid interaction assays using liposome microarrays, incorporating various analytical methods, such as fluorescence microscopy, surface plasmon resonance, and atomic force microscopy. Critical parameters and metrics for data analysis, such as ...
The Protein Lipid Overlay (PLO) assay enables the identification of the lipid ligands with which lipid binding proteins interact. This assay also provides qualitative information on the relative affinity with which a protein binds to a lipid. In the PLO assay, serial dilutions of different lipids...
The C-terminal tail of MlaA is an important part of the MlaC–MlaA interaction, although other parts of the interface, such as the ɑ7 helix, may also play a role in binding and lipid transfer. In crystal structures of free MlaA–OmpF/C complexes, the electron density is not observed ...
lipid environment. Here, we demonstrate that ZIKV does not require cholesterol for replication in mosquito cells (Supplementary Fig.8), but cholesterol is incorporated into the lipid envelope most likely via its interaction with the membrane resident M-domain of prM when ZIKV is grown in mammalian...
exposure of the protein surface in solution and the low refolding rate of recombinant proteins in target cells remain important obstacles for the intracellular delivery of therapeutic proteins5. Several lipid nanoparticle-mediated protein delivery methods have been postulated, as has protein encapsulation ...
Moreover, in vivo protein-protein interaction assays revealed that SPL7 is able to homodimerize, probably mediated by the IRPGC domain. These observations, in combination with the constitutive activation of SPL7 targets, when ectopically expressing the N-terminal part of SPL7 including the SBP ...
protein-protein interaction DBD: DNA binding domain AD: activating domain CAPPIA: cell array protein-protein interaction assay PRB: prey, reporter, bait PR prey: reporter R1881: methyltrienolone MPA: medroxyprogesterone acetate OH-Flu: hydroxyflutamide AR: androgen receptor AR-LBD: ...
In H. mediterranei this assay allowed the study the interaction of a PPS-like protein and the promoter region of the PHA synthase that catalyzes the PHBV [poly(3-hydroxybutyrate-co-3-hydroxyvalerate)] polymerization [99]. In H. volcanii has been used for the study of the Replication Protein...