22 kDa PI of ligand 4.69 Degree of substitution Approximately 2 mg protein G/ml settled resin Static binding capacity > 20 mg sheep IgG/ml settled resin Matrix spherical 4% cross-linked agarose Average particle size 90 μm (45-165 μm) Storage solution 1X PBS containing 20% ethanol ...
Regeneration conditions were unacceptable for Kelch protein stability, requiring a new surface for each SPR experiment. However, the successful generation of murine Keap1(322–624)-C-10His via EPL enabled SPR surface regeneration (Fig. 3c) resulting in a ~10-fold increase in assay throughput (...
regeneration Processes of repair or replacement of missing structures. regulatory sequence DNA sequence (control element) to which regulatory molecules such as promotors or enhancers bind, thereby altering the expression of the adjacent gene. regulatory T-cell See T-regulatory cells. A situation in wh...
Both react with primary amines, but AminoLink™ Coupling Resin is a two-step conjugation. First a Schiff base is formed between the amine and the aldehyde on the resin, which is then reduced to a stable sec...
Sustainable nitrate-contaminated water treatment using multi cycle ion-exchange/bioregeneration of nitrate selective resin The sustainability of ion-exchange treatment processes using high capacity single use resins to remove nitrate from contaminated drinking water can be achi... S Ebrahimi,DJ Roberts -...
Resin lifetimeEffective Protein A column regeneration is important in therapeutic monoclonal antibody (mAb) production for product quality and process economics. To design a robust and effective regeneration strategy, resin compatibility, microbial inactivation efficiency and cleaning effectiveness are three ...
Regeneration step should be operated after elution to remove the residue proteins and impurities on the resin for repeated use. Wash the resin with 2–3 bed volumes of regeneration buffer followed by re-equilibration with 2–3 bed volumes of binding buffer. Sometimes the regeneration buffer is ...
interaction between OsHOX3 and the promoter ofOsPYL5. -AbA, 0 ng/mL; +AbA, 200 ng/mL.iChIP-qPCR assays. Enrichment of OsHOX3 inOsPYL5promoter. The upper schematic indicates the locations of the DNA fragments used for ChIP-qPCR. The red square indicates the element. A/T rich ...
The reactions contained 3.0 µM LarA and 0.125 µM Lon-His hexamer with (+ATP) or without (–ATP) ATP regeneration system (ATP, creatine phosphate, creatine kinase [CK]). f Quantifications showing LarA protein levels (normalised to Lon-His levels) as mean values ± SD; n...
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