Highly potent analogue of capsaicin from Euphorbia resinifera (flowering cactus) that is an agonist at vanilloid receptor-1. Research article: http://www.molecularpain.com/content/4/1/3 An adipokine (cysteine-rich secreted protein FIZZ3 (found in inflammatory zone 3), 108aa produced by adipocy...
High protein diet complements resin therapy of familial hypercholesterolemia. Clin Invest Med. 1992;15:349-359Google Scholar 21. Wolfe BM, Piche LA. Replacement of carbohydrate by protein in a conventional-fat diet reduces cholesterol and triglyceride concentrations in healthy normolipidemic subjects...
Aliquots of lysates containing 100 μg of total protein were processed according to the multi-enzyme digestion FASP protocol (18). Briefly, protein lysates were depleted from the detergent using 8 m urea in 0.1 m Tris/HCl, pH 8.5, thiols were alkylated with iodoacetamide, and proteins were co...
Proteins were purified by Ni-NTA-affinity resin (Qiagen). Purity and monomeric state were confirmed using a Superdex 75 increase 10/300 GL column (GE Healthcare) and SDS-PAGE gels. Size exclusion chromatography (SEC) data reported is immediately post-Ni-NTA purification without additional ...
ProPlus PhyTip column tips (Biotage, PTV-92-20-07) were loaded on the Bravo 96 LT head and equilibrated by aspirating and dispensing 75 μl of PBS, repeating four times. Sample binding to the tip resin was performed by aspirating and dispensing 98 μl of harvested supernatant, ...
The cleaved proteins were separated by passing through Ni-Sepharose resin and further purified using size-exclusion chromatography (Superdex S-200). The resultant pure recombinant HSP90 was stored at −80 °C in a buffer containing 30 mM HEPES/NaOH, pH 7.5, 150 mM NaCl, 1 mM TCEP...
Purify the modified protein from reaction byproducts by dialysis using a membrane with a low-molecular-weight cutoff or gel filtration using desalting resin and a buffer consisting of 50-mM sodium phosphate, 0.15-M NaCl, 10-mM EDTA, pH 7.2. The SPDP-activated phycobiliprotein may be reacted...
Afterwards, the NaCl concentration of the cleared lysate was adjusted to 500 mM and the cleared lysate was applied to 10 mL (=5 mL bed volume) equilibrated Talon SuperFlow Metal Affinity Resin (TaKaRa) per L culture on ice for 45 min. Subsequently, beads were washed 5 times with...
Tissue homeostasis is maintained after stress by engaging and activating the hematopoietic stem and progenitor compartments in the blood. Hematopoietic stem cells (HSCs) are essential for long-term repopulation after secondary transplantation. Here, usin
respectively. The printer was calibrated following the manufacturer’s protocol. The layer thickness was set to 25 µm and the printing build style was provided by the manufacturer and was specific to the printing material. We selected HTM140V2 (EnvisionTEC, Germany) resin owing to its high...