In the cell, as in vitro, the final conformation of a protein is determined by its amino-acid sequence. But whereas some isolated proteins can be denatured and refolded in vitro in the absence of other macromolecular cellular components, folding and assembly of polypeptides in vivo involves othe...
自2010年创刊以来,《Protein & Cell》始终秉承主编团队的办刊理念,坚持全球开放获取(Global Open Access)出版,坚持及时公正地为全球学者尤其是青年学者的最新科研成果提供绿色通道(Green Channel for Rising Stars in Science);同时,做到公平公正,在世界范围内积极推动生命科学发展,促进人类科技进步。 THE END IF: 13.6...
Homeostasis of protein concentrations in cells is crucial for their proper functioning, requiring steady-state concentrations to be stable to fluctuations. Since gene expression is regulated by proteins such as transcription factors (TFs), the full set of proteins within the cell constitutes a large s...
自2010年创刊以来,《Protein & Cell》始终秉承主编团队的办刊理念,坚持全球开放获取(Global Open Access)出版,坚持及时公正地为全球学者尤其是青年学者的最新科研成果提供绿色通道(Green Channel for Rising Stars in Science);同时,做到公平...
PROBLEM TO BE SOLVED: To provide a method for determining quantitatively the number of molecules in a target protein existing in a cell of a measuring object.今本 文男曽根 岳史吉田 尚平内田 浩二
Nat Rev Mol Cell Biol (2024) The State-of-the-Art Overview to Application of Deep Learning in Accurate Protein Design and Structure Prediction Saber Saharkhiz, Mehrnaz Mostafavi, Amin Birashk, Shiva Karimian, Shayan Khalilollah, Sohrab Jaferian, Yalda Yazdani, Iraj Alipourfard, Yun Suk Huh...
mCAT-1 transfer in human cells by mCAT-1 gesicles. (a) Production process of mCAT-1 vesicles (b) Surface staining of mCAT-1 on gesicle-treated cells. Human cell lines treated by mCAT-1 gesi... Figure S2. Gesicle-mediated transfer of mCAT-1 depends on endosomal acidification. The mCAT-...
How to choose the right protein gel for your research application This webinar will highlight the different protein modifications occurring during electrophoresis, the importance of pH in sample integrity, how to choose the right gel chemistry for specific research applications, and how to introduce th...
Tumor protein p53 was co-transfected to verify the functions of miR-151a-3p. The miR-151a-3p level in NPC tissues was much higher than that in adjacent tissues. After transfecting cells with miR-151a-3p mimic, the cell proliferation and patients' survival rate were much increased, and ...
Cryo-electron tomography is a major tool used to study the structure of protein complexes in situ. However, the throughput of tilt-series image data collection is still quite low. Here, we show that GisSPA, a GPU accelerated program, can translationally