Protein functions in the cell, such as enzymatic activity, signaling, and transport, are driven by conformational changes between multiple states1,2,3. To understand biological processes at the molecular level, we therefore require a precise description of protein dynamics1. Pump-probe time-resolved ...
Proteins that are linked to or embedded in the cell membrane may associate with other proteins (protein–protein interactions) either on the inner or outer leaflet of the membrane. Other components of the membrane, mainly lipids, are essential or can regulate the function of some of these ...
Protein functions in the cell, such as enzymatic activity, signaling, and transport, are driven by conformational changes between multiple states1,2,3. To understand biological processes at the molecular level, we therefore require a precise description of protein dynamics1. Pump-probe time-resolved ...
They are sometimes anchored to lipids in the membrane or bound to other membrane proteins (Figure 5). Figure 5: The fluid-mosaic model of the cell membrane Like a mosaic, the cell membrane is a complex structure made up of many different parts, such as proteins, phospholipids, and ...
The amino acid chain of an integral membrane protein can pass through the cell membrane once, or weave in and out of the cell membrane multiple times. They contain non-polar side chains, which allow them to interact with the fatty acid chains of the phospholipids. This interaction holds the...
functional studies at atomic resolution have also become possible for largeintegral membrane proteins. Solution NMR spectroscopy provides unique insights complementary to other structural methods and can contribute significantly to elucidate mechanisms of membrane protein function and related biology in general....
In order to establish protein array technology for the characterization of the weak interactions between leukocyte membrane proteins, we selected the human leukocyte membrane protein CD200 (OX2) and its cell surface receptor (hCD200R) as a model system. As antibody-antigen reactions are generally ...
LIF displays diverse biological effects, but is best known for its ability to inhibit the differentiation of embryonic stem cells in mice and contribute to stem cell self-renewal.Human and mouse LIF share 79% sequence homology and exhibit cross-species activity. However, LIF inhibition of stem ...
Chemical probes are selective small-molecule modulators, usually inhibitors, of their target protein’s function, that can be used in cell or even animal studies to interrogate the functions of their target proteins. Cheryl Arrowsmith, the leader of a new initiative called Target 2035, which seeks...
A temperature-inducible protein module for control of mammalian cell fate The Melt (Membrane localization using temperature) protein translocates to the plasma membrane upon temperature shift. Melt variants with a range of switching temperatures enable straightforward thermogenetic control of diverse cellular...