Protein half-life The time required for the amount or concentration of a protein to be reduced by 50% under physiological conditions. It is a measure of the propensity of a protein to be degraded by the ubiquitin–proteasome system (UPS). Proteins with short half-lives are rapidly degraded ...
The half-life of retinol-binding protein is about 10 hours and, as with serum prealbumin, it has a small body pool and is a more specific marker of subtle changes in protein stores. Similar to serum albumin and serum prealbumin, retinol-binding protein levels decrease during inflammatory ...
AMG132 stabilizes TWIST1.BKnock-down of SPOP prolongs the half-life of TWIST1 protein. Cell lines transfected with shCtrl or shSPOP (sh67) vectors were treated with 20 µg/ml CHX and whole protein lysates were collected at the indicated times for western blotting.CTWIST1 protein level ...
Life - DNA, RNA, Protein: The specific carrier of the genetic information in all organisms is the nucleic acid known as DNA, short for deoxyribonucleic acid. DNA is a double helix, two molecular coils wrapped around each other and chemically bound one to
photobleaching experiments demonstrated that occludin and ZO-1 rapidly enter and leave tight junctions (Shen et al., 2008). Occludin has a short half-life of 1.5 h inMDCK cells(Traweger et al., 2002) and cycles among endosomal populations includinglysosomes(Ivanov et al., 2004). Its subcell...
AI-Driven Efficient De Novo design of GLP-1RAs with Extended Half-Life and Enhanced Efficacy Ting Wei, Xiaochen Cui, Jiahui Lin, Zhuoqi Zheng, Taiying Cui, Liu Cheng, Xiaoqian Lin, Junjie Zhu, Xuyang Ran, Xiaohun Hong, Zhangsheng Yu, Haifeng Chen bioRxiv 2025.03.26.645438 • ProteinMPN...
(unpublished data). Thus, there is a balance between sheer potency, which can be achieved with small size, and long half-life, which typically brings with it greater size and less potency. Additionally, both the size of the cell surface receptor of the target cells and the epitope to ...
up to 120 days and stable albumin adducts a half-life of 20–25 days (reviewed in Sabbioni and Jones2002; Skipper and Tannenbaum1990; Törnqvist et al.2002)) in humans. Reaction products with hemoglobin accumulate up to 60 times a single daily dose and albumin adducts up to 29 times a...
On a third dataset containing only mRNA-protein interactions, the performance of RPISeq was competitive with that of a published method that requires information regarding many different features (e.g., mRNA half-life, GO annotations) of the putative RNA and protein partners. In addition, RPISeq ...
(2)-C cells were transfected with an empty vector or lncNB1 expression construct, followed by treatment with vehicle control or cycloheximide. The pulse chase assays showed that N-Myc protein half-life was reduced by approximately 50% by lncNB1 siRNA or DEPDC1B siRNA (Fig.3c), and was ...