Protein G Magnetic Beads蛋白G磁珠 1ml(50mg/ml) XG-P2767 产品介绍: Protein G Magnetic Beads 是大小均匀,具有极好分散度的,表面共价缀合超纯(纯度>97%)的重组蛋白 G 的二氧化硅基质超顺磁珠。这种磁珠是经过专门设计,并经过严格质量管理检测的,主要用于当使用的一级抗体确定时,用于免疫沉淀和细胞分选。 同...
ProteinGBeads 货号规格 BDTL0003-11ml BDTL0003-55ml BDTL0003-2525ml BDTL0003-100100ml 1.产品介绍 蛋白G层析介质是用于分离和纯化lgG的亲和层析介质,具体性能见下表。ProteinG 是一种分离自GStreptococci的细胞壁蛋白,它可通过其Fc片段结合哺乳动物IgG。重组 proteinG含有高亲和结合位点,减少了非特异性吸附。
抗体名:SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) 靶点:详见说明书 宿主:详见说明书 用途:公司产品仅用于科研 应用范围:公司产品仅用于科研 纯度:详见说明书 % 产地:进口、国产 品牌:谷研 货号:GOYK97516 用途:仅用于科研 亚型:IgG ...
Dynabeads Protein G beads are uniform, 2.8-μm superparamagnetic beads with recombinant Protein G (∼17 kDa) covalently coupled to the surface. Dynabeads Protein G beads provide a great alternative to Sepharose resin or agarose resin for immunoprecipitation (IP) combined with your own antibody, an...
3去除或减少⾮特异性背景：如免疫沉淀技术中在加⼊特异性抗体前加proteinA/Gbeads到细胞裂解液中，以减低背景。蛋⽩A、蛋⽩G与不同的免疫球蛋⽩的结合能⼒并不⼀样，它们受到后者的来源及亚类的影响。proteinAagarose和proteinGagarose之间主要的区别是什么？它俩跟免疫球蛋⽩结合的机理？ProteinA:天...
Dynabeads Protein G beads are uniform, 2.8-μm superparamagnetic beads with recombinant Protein G (∼17 kDa) covalently coupled to the surface. Dynabeads Protein G beads provide a great alternative to Sepharose resin or agarose resin for immunoprecipitation (IP) combined with your own antibody, an...
(B) The majority of proteins show up only in the heavy form (infinite ratio (Inf), n = 3034) with few exceptions that were exclusively identified in the light form (-Inf, n = 47). Light proteins included proteins with very few peptides identified and extracellular proteins (e.g., seru...
2 g rice seedlings were grinded with liquid nitrogen and fixed with 1% formaldehyde under vacuum. After nuclei were isolated and lysed, chromatin was ultrasonic fragmented on ice to an average size of 500 bp. The supernatant was firstly blocked with protein A agarose beads pre-absorbed with...
Peptides were loaded on a pre-column (75 μm ID, 4 cm long, packed with ODS-AQ 120 Å–10 μm beads) and separated on an analytical column (75 μm ID, 12 cm long, packed with Luna C18 1.9 μm 100 Å resin). Slight modifications to the separation method were made ...
Beads were incubated overnight at 65 °C in 100 μl of Elution Buffer (50 mm Tris-HCl, pH 8.0, 1 mm EDTA, 1% SDS, 50 mm NaHCO3, 0.2 m NaCl). De-cross-linked DNA was treated with 40 μg/ml RNase A (Qiagen, Dusseldorf, Germany) and 20 μg/ml proteinase K (Sigma) and ...