Dynabeads Protein G beads are uniform, 2.8-μm superparamagnetic beads with recombinant Protein G (∼17 kDa) covalently coupled to the surface. Dynabeads Protein G beads provide a great alternative to Sepharose resin or agarose resin for immunoprecipitation (IP) combined with your own antibody, an...
Dynabeads Protein G beads are uniform, 2.8-μm superparamagnetic beads with recombinant Protein G (∼17 kDa) covalently coupled to the surface. Dynabeads Protein G beads provide a great alternative to Sepharose resin or agarose resin for immunoprecipitation (IP) combined with your own antibody, an...
•See magnetsfor Dynabeads magnetic beads separations •KingFisher automation protocols •Watch a video about the KingFisher Flex instrument *Sepharose is a trademark of GE Healthcare Bio-Sciences AB. 仅供科研使用。不可用于诊断程序。 规格
The lysates were cleared by centrifugation at 9000 × g for 30 min at 4 °C and used immediately for the binding assays. The His-tagged Xph clones lysates were added to 40 µL of His Mag Sepharose excel magnetic beads (GE Healthcare) that had been first washed twice with ...
Cell lysates were incubated with specific antibodies overnight at 4 °C and then incubated for 3 h with protein A/G-Sepharose beads (GE Healthcare, Uppsala, Sweden). Duolink proximity-ligation assay The endogenous interaction of Rab5 and Gapex5 was observed using a Duolink kit (Sigma,...
2 g rice seedlings were grinded with liquid nitrogen and fixed with 1% formaldehyde under vacuum. After nuclei were isolated and lysed, chromatin was ultrasonic fragmented on ice to an average size of 500 bp. The supernatant was firstly blocked with protein A agarose beads pre-absorbed with...
Beads (50 μL slurry) were equilibrated 3x with wash buffer and then incubated with 6xHis-TRIM11 or Hsp70 (1 μg each) at 4°C for 3 h with slight agitation. The beads were then washed 3x with wash buffer, and the bound proteins were eluted with elution buffer. Input and bound ...
The homogenate was centrifuged at 8000 g for 10 min at 4°C to remove the nuclei and Mito. For immunoisolation method, protein A/G agarose beads pre-bound with anti-Rab5 (EE marker)/anti-Rab7 (LE marker) antibodies were used to pull down EE/LE in the supernatant, respectively [...
Then, 50 μL of equilibrated Protein G Mag Sepharose Xtra (GE Healthcare) magnetic beads were added to extracts and rotated overnight at 4°C. Finally, beads were recovered, washed three times with 1 mL CoIP Buffer supplemented with NaCl 300 mM, Triton X-100 0.1% (v/v) and cOmplete ...
Approximately 100 μl glutathione-coupled sepharose beads (GE Healthcare) were equilibrated with a buffer containing 20 mM TRIS (pH 8.0), 100 mM NaCl and 5 mM DTT and were incubated with cleared and filtered lysate from 1 l bacterial expression culture for 1 h at 4 °C. ...