Chemical probes are selective small-molecule modulators, usually inhibitors, of their target protein’s function, that can be used in cell or even animal studies to interrogate the functions of their target proteins. Cheryl Arrowsmith, the leader of a new initiative called Target 2035, which seeks...
The ability to analyze protein function in a native context is central to understanding cellular physiology. This study explores whether tagging endogenous proteins with a reporter is a scalable strategy for generating cell models that accurately quantit
The core function of PDI-like protein members is to facilitate the formation of disulfide bonds for secretory and membrane proteins entering the SP, particularly in the ER [26,27,28,29]. This ensures that the client proteins are in a proper stabilization and maturation state prior to ER exit...
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and they carry out important and diverse functional roles in the cell directly facilitated by their lack of structure. Despite their prevalence and critical roles in function and disease, IDRs do not fit neatly in the structure-function paradigm and remain outside the capabilities of structure-based...
Inducible inactivation of a protein is a powerful approach for analysis of its function within cells. Fission yeast is a useful model for studying the fundamental mechanisms such as chromosome maintenance and cell cycle. However, previously published strategies for protein-depletion are successful only ...
Onco-MLL1 proteins contain MLL1 (1– ~ 1400) merged with a variety of fusion partners (Fig.1a, b). The main function of MLL1 portion is to recognize and bind MLL1-target genes with a high affinity through multivalent interactions involving MLL1-Menin-LEDGF, ATH, and CxxC. This ...
37 Inhibiting the LAG-3 and PD-1 pathways could improve T-cell function in ovarian cancer.66 In hepatocellular carcinoma, elevated expression of LAG-3 on TILs has been observed.67 In gastric cancer, positive PD-1 and LAG-3 expression was elevated on CD4-positive and CD8-positive T cells,...
This is apparent from a recent publication by Jovanovic et al. (2015). The authors showed that mRNA raw data directly could explain 27% of the gene-to-gene variation of protein levels in cells before LPS stimulation. When modeling protein levels as a function of transcript concentration, ...
and then construct a sequence of subgraphs, where each subgraph is a subset of the next one. They then define a permutation-invariant descriptor function for each subgraph that is related to curvature. This function is designed to be invariant to the order in which the nodes are arranged, so...