MS BLAST running with this parsing script has been installed at a web-accessible server (dove.embl-heidelberg.de/Blast2/msblast.html). Modified FASTA-based algorithms, such as FASTS and FASTF (14, 16, 17), evaluate hits by original scoring procedures and statistical significance criteria. ...
Protein phosphatase PP1 negatively regulates the Toll-like receptor- and RIG-I-like receptor-triggered production of type I interferon by inhibiting IRF3 phosphorylation at serines 396 and 385 in macrophage Cell Signal., 26 (12) (2014), p. 2, 10.1016/j.cellsig.2014.09.007 930-2 939 Google...
αReps are a family of artificial repeat proteins2,22 composed of a HEAT-like motif23. Each protein member of the αRep library has the same general architecture but is endowed with a unique binding surface made by the juxtaposition of hypervariable residues24. Specific αRep binders for a ...
Plates were then centrifuged at 700 × g for 10 s and placed on a magnetic rack (Alpaqua Magnum FLX) where 1.7 µl from each well was transferred for protein analysis. The Smart-seq2 and SPARC samples were supplied with 6 µl and 10 µl reverse transcription mix, ...
ostertagi peptides with the most prevalent domain being ‘NAD (P)-binding’ domain (Table 2). In the free-living stages, globin, zinc finger domains, and chromo domains were among the most prevalent (Figure 4 and Additional files 17 and 18). In the parasitic stages, metridin-like ShK ...
constructed by de novo assembly of gilthead sea bream sequences derived from public repositories of mRNA and collections of expressed sequence tags together with new high-quality reads from five cDNA 454 normalized libraries of skeletal muscle (1), intestine (1), head kidney (2) and blood (1...
Here we report the identification of mixed lineage kinase domain-like, MLKL, as a key RIP3 downstream component of TNF-induced necrosis. Through screening a kinase/phosphatase shRNA library in human colon adenocarcinoma HT-29 cells, we found that knockdown of MLKL blocked TNF-induced necrosis. ...
Fluoxetine (FLX), a typical drug belonging to the category of selective serotonin reuptake inhibitors (SSRI), is the most widely prescribed psychoactive dr... X Jing - 《Arteriosclerosis Thrombosis & Vascular Biology》 被引量: 2发表: 2017年 Differential induction of apoptosis by antidepressants in...
CAP1flx/flx/CAP2−/−and CAP1flx/flx/CAP2+/-embryos and transfected them at DIV6 with GFP together with either catalytically active mCherry-tagged Cre recombinase (Cre) or a catalytically inactive mCherry-Cre (Cre-mut). We thereby obtained (i) Cre-expressing CAP1flx/flx/CAP2−/...
For constructing pSTD241, a 3.9-kilobase KpnI-EcoRI fragment containing thehflX-hflK-hflC9 genes was first cloned into pSTV29 (Takara Shuzo); then, the HindIII secY fragment of pKY3 (21) was inserted into the HindIII site on the vector. Table I. Bacterial strains and plasmids Empty ...