Protein Extraction beads C20000020 将 Protein Extraction kit for Bioruptor® Plus 添加至我的购物车。 结账 继续购物 × Protein Extraction kit for Bioruptor® Plus B01200013 将 15 ml tube holder for Bioruptor® Standard & Bioruptor® Plus 添加至我的购物车。 结账 继续购物 × 15...
For peptide mixture purification (desalting) and terminal peptide enrichment, lab-made stop-and-go-extraction tips (StageTips) are used (Rappsilber et al., 2007). StageTips are ordinary pipette tips packed with small membrane incorporating chromatographic sorbents (Figure 2). In this protocol, 20...
Aliquots of lysates containing 100 μg of total protein were processed according to the multi-enzyme digestion FASP protocol (18). Briefly, protein lysates were depleted from the detergent using 8 m urea in 0.1 m Tris/HCl, pH 8.5, thiols were alkylated with iodoacetamide, and proteins were co...
To compare the computation required for each MSA, we compared the time it took to generate MSAs for three protein pairs (PDB: 4G4S_P-O, 5XJL_A-2 and 5XJL_2-M), using either the block diagonalization or AF2 protocol. The tests were performed on a computer using 16 CPU cores from an...
It is targeted to selectively match the neutron scattering length density (NSLD) of the solid matrix and allow the extraction of structural parameters to determine the overall structure of biomacromolecules from a multi-component system32,33. The scattering intensity is proportional to the square of ...
(3) The reference NPCL nucletide and peptide sequences can be found in the folder"6.Reference_sequences_for_target_regions". These two seqeunce files will be used in the extraction of orthologous sequence groups from assembled contigs. For detailed information, please refer to##A part of ...
2.2. Protein extraction The protein extract was prepared from tubers by mechanical homogenization (mortar and pestle) in liquid nitrogen. The resulting powder was dissolved in 0.1 M Tris-Cl buffer, pH 8.0, containing 10% glicerol (extract:buffer ratio was 1:1). The tuber extract (50% v/v...
to three cycles of flash-freezing in liquid nitrogen, followed by thawing at 37 °C for extraction of soluble protein. After the final thawing, samples were pelleted using a refrigerated tabletop centrifuge for 30 min at 17,000gto separate soluble protein from insoluble cellular debris. The...
Two-dimensional (2D) protein films can be used to modify the properties of surfaces, and find applications predominantly in the fields of biomaterials, lithography, optics and electronics. However, it is difficult to produce scalable homogeneous and robu
At the proteomic level, the cell-type-resolved proteome in mouse liver was uncovered by the comprehensive profiling of 11,520 proteins using an isolation protocol based on the particular density and immunogenic properties of different hepatic cell types. The clustering analysis of the quantified ...