Olena Z, Christina G, Louise A, Serhiy S (2011).Optimized protocol for protein extraction from the breast tissue that is compatible with two- dimensional gel electrophoresis. Breast Cancer. 5:37-42.Zakharchenko O, Greenwood C, Alldridge L, Souchelnytskyi S. Optimized protocol for protein ...
SDS extraction followed by acetone precipitation – simple extraction protocol that does not require phenol.Recommended start protocol for whole tissue extractions. 1.Grind 1 g of fresh tissue to a powder with liquid nitrogen in a mortar and pestle. 2.Add 5 ml of extraction media (0.175 M Tri...
Protein extraction from tissues and cultured cells is the first step for many biochemical and analytical techniques (PAGE, Western blotting, mass spectrometry, etc.) or protein purification. Efficient disruption and homogenization of animal tissues and cultured cells are required to ensure high yields ...
化学名:Tissue protein rapid extraction kit (spin column method)CAS番号. 英語名:Tissue protein rapid extraction kit (spin column method)CBNumberCB55814801MW0MOL FileMol fileTissue protein rapid extraction kit (spin column method) 生産企業 Send Inquriy ...
bodily fluids such as urine or plasma are already more or less homogeneous protein solutions with low enzymatic activity, and only minor manipulation is required to obtain proteins from these samples. Tissue samples, however, require extensive manipulation to break up tissue architecture, control enzyma...
Tissue cell lysis protein yield with T-PER Tissue Protein Extraction Reagent 规格 形式:Liquid 试剂类型:Tissue Extraction Buffer, Detergent Solution, Cell Lysis Buffer 内容及储存 Store at room temperature. 公司简介 北京杰辉博高生物技术有限公司依托*重点实验室建立,是由具有资深生命科学、生物技术背景及留学...
Protein extraction from human tumor tissues of ovarian cancer patients and patient information All tissue specimens were obtained from the Duke Gynecology Oncology Tissue Bank (Durham, NC, USA), with patient consent and were used under a protocol approved by the Duke Institutional Review Board. Froze...
cDNA extraction from the tissue lysate was performed in two steps. In the first step, all DNA was extracted from the lysate using the DNA purification kit (Zymo Research, ZD4014). We followed recommended protocols using a 5:1 ratio for the DNA binding buffer and lysate. In the second step...
Tissue protein rapid extraction kit (spin column method) Basic information More.. Product Name: Tissue protein rapid extraction kit (spin column method) Synonyms: CAS: MF: MW: 0 EINECS: Mol File: Mol File Information Error Report Your Email: Browse by Nationality Tissue protein rapid ...
Protein extraction from cellular compartments using T-PER Tissue Protein Extraction Reagent. Lysates from prepared using T-PER Reagent and extraction efficiency from the various cellular compartments of different tissues compared. For each target protein, 10µg of lysate was loaded for and electrophore...