After detecting by SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gelelectrophoresis) electrophoresis, compared with the protein band obtained by other two representative extraction methods of the soil protein, the band of the soil protein extracted by the method is clearer.李乃荟...
We conclude that the dependence of protein recovery on both extractant and soil type prevents direct comparison of studies using different recovery methods, particularly if no extraction controls are used. We present recommendations for a standard protein extraction protocol....
An unusual and abundant protein was found on hyphae of AMF, and it was hypothesized that the hyphal protein could be found in soil. The purpose of this work was to test extraction conditions for soil protein on a range of soils collected from undisturbed sites, to compare protein from ...
名称Soil Protein Extraction Kit / 土壤蛋白提取试剂盒 规格50T | 100T 存储 蛋白酶抑制剂-20℃保存;蛋白提取液室温保存。有效期:一年。 试剂组分 Component 50 assay 100 assay Reagent A: Soil Protein Extract(土壤蛋白提取液) 50ml 100ml Reagent B: Protease Inhibitor Cocktail(蛋白酶抑制剂混合物) 100ul...
metagenomic DNA, RNA, isolationsoil RNA isolationmetatranscriptomicsThis chapter contains sections titled: Introduction Methods for Soil DNA Isolation Methods for Soil RNA Isolation Simultaneous Extraction of RNA and DNA Isolation of Plasmid DNA from Soil Isolation of the Soil Metaproteome Conclusion ...
The soil protein was mainly from 138 families, 346 species of microorganisms. This optimization method could obtain more proteins than previous methods, with a wider range of molecular weight and pI. This study lays an important foundation for the research and development of soil metaproteomics....
Extraction of inulin from Jerusa... H Yi,L Zhang,C Hua,... - 《Food & Bioprocess Technology》 被引量: 46发表: 2010年 Characteristics of Isolation and Functionality of Protein from Tomato Pomace Produced with Different Industrial Processing Methods The seeds separated from tomato pomace may ...
Methods Bacterial strain and growth conditions R. qingshengiiS10107 (JX173283) was originally isolated from the rhizospheric soil ofPhaseolus vulgarisL. (Red Kidney Bean, RKB) from Chhiplakot region (3,290 m, 30.06 °N, 79.01 °E) of Kumaun Himalayas on the Burk’s Medium (nitrogen deficient...
Material and Methods Construction of metagenomic library and functional screening Soil samples were collected from Jundiaí Riverbanks (Natal, Brazil), which were characterised by high salinity (description of collecting point is in Supplementary Table S1). An overview of the steps and procedures used ...
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