Methods for producing a protein extract from cells, such as cells containing viral proteins, are provided. In general terms, the methods involve: increasing the pH of the cells to a pH of at least about pH 10.0 to produce an intermediate composition, and then, in the presence of a non-...
Method Of Protein Extraction From Cells 发明人:Stephen Lovell,Lydia Blank,Virginia Crews,Nancy Hasse,Peter Lu,Johannes Schweizer 申请号:US1174 7830 申请日:20070511 公开号:US20070292899A1 公开日:20071220 摘要:Methods for producing a protein extract from cells, such as cells containing viral proteins,...
Protein extraction from cultured cells Approximately 1–5 g (FW) of Cj156-S and CjY cells were inoculated in 20 or 100 ml of culture medium and cultured for 1–2 weeks. The grown cells were collected and ground in liquid nitrogen. An equal volume of extraction buffer [50 mM Tris...
Protein extraction from tissues and cultured cells is the first step for many biochemical and analytical techniques (PAGE, Western blotting, mass spectrometry, etc.) or protein purification. Efficient disruption and homogenization of animal tissues and cultured cells are required to ensure high yiel...
which were comparable to those of systems obtained from fast-growing cells. Our approach is less laborious than traditional extract preparation methods and multiplies the yield of extract per cultivation. This simplified growth protocol has the potential to attract new entrants to cell-free protein syn...
palmata protein extraction using osmotic shock, high shear force, alkaline and polysaccharidase treatments and combinations of these parameters. 2. Material and methods 2.1. Materials Celluclast® 1.5L, Shearzyme® 500L and Ultraflo® L were kindly provided by Novozymes (Bagsvaerd, Denmark)...
The SOD protein standard was prepared by extraction from E. coli cells with Marathon A2 microparticles. Cells were incubated for 2 h at ratio 0.7 v/v (resin:cells) in 50 mM TRIS buffer at pH 8.0. Protein was desorbed with 0.2 M NaCl and subsequently purified by gel filtration (Superdex...
Total protein quantification methods Protein concentration quantitation is an integral part of any laboratory workflow involving protein extraction, purification, labeling or analysis. Pierce Protein Assays provide a wide range of options for ...
Protein extraction from brain tissue and neuroblastoma cell line The brain tissues were thawed on ice and homogenized using Ultra-turrax T8 homogenizer (IKA-Werke GmbH & Co. KG, Germany) in solubilization buffer containing 8M urea, 2% (w/v) CHAPS (3-[(3-cholamidopropyl)dimethylammonio]-1-pr...
Cells were solubilized with lysis buffer (8 M urea and 4% (w/v) SDS in 50 mM Tris–HCl, pH 7.5) and incubated at 95 °C for protein extraction. Cell lysates were sonicated on ice using the Bioruptor UCD-250T (Cosmo Bio, Tokyo, Japan). The solution was subjected to chlorofor...