Protein in our diets comes from both animal and vegetable sources. Most animal sources (meat, milk, eggs) provide what's called "complete protein", meaning that they contain all of the essential amino acids. Vegetable sources usually are low on or missing certain essential amino acids. For ex...
human tissue plasminogen activator (t-PA) from a corresponding endogenous protein of the host cell, e.g. Chinese hamster t-PA, is disclosed. The method comprises contacting a fluid containing the recombinant protein with antibodies specifically binding the corresponding endogenous protein. Monoclonal ...
IP of Flag®-tagged proteins from a cellular extractA typical IP consists of five steps:Preparation of cell lysate Binding of the antibody to the Flag®-tagged protein Washing off unbound molecules Elution of the Flag®-tagged protein Detection and analysis of the Flag®-tagged protein on...
Transcriptional bursting has been linked to the stochastic positioning of nucleosomes. However, how bursting is regulated by the remodeling of promoter nucleosomes is unknown. Here, we use single-molecule live-cell imaging of GAL10 transcription in Sacch
The adaptor Grb7 is a novel calmodulin-binding protein: functional implications of the interaction of calmodulin with Grb7 Oncogene, 24 (26) (2005), pp. 4206-4219, 10.1038/sj.onc.1208591 View in ScopusGoogle Scholar [60] R.J. Daly The Grb7 family of signaling proteins Cell. Signal., 10...
Some soluble, misfolded proteins can bypass the refolding action of chaperones in vivo according to folding and functional assays1,2,3. Typically, in these assays the protein of interest is purified after it has been expressed either heterologously or constitutively from different synonymous messenger ...
What protein should I use to block a transfer membrane for a western blot? My name is Srikanth, I'm a Product Scientist at Cell Signaling Technology, and this is CST Tech Tips. In regards to western blotting, a common question we always get is, what should I use, milk or BSA to blo...
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The fetal versionbinds more strongly to oxygen, allowing a fetus to gather enough oxygen from its mother's bloodstream. The adult version normally takes over after birth, once oxygen can be obtained through breathing. However, in sickle cell disease and beta thalassemia, people have faulty version...
Mashing the banana exposes a greater surface area from which to extract the DNA. The liquid soap is added to help break down cell membranes to release the DNA. The filtration step (pouring the mixture through the strainer) allows for the collection of the DNA and other cellular substances. ...