Recombinant histidine-tagged xPNAS-4 protein was expressed in Escherichia coli as insoluble inclusion bodies. The inclusion bodies were subsequently dissolved in 8 M urea and purified to near homogeneity by Ni2+ affinity chromatography. The resulting denatured protein was refolded by stepwise dilution ...
(1) Inclusion bodies within cells, often of virus particles, although this term is more common in the older literature. (2) Infectious extracellular form of Chlamydia, consisting of electron-dense nuclear material and a few ribosomes surrounded by a rigid trilaminar wall. Once taken up by cell...
Protein expression in E. coli is the most commonly used system to produce protein for structural studies, because it is fast and inexpensive and can produce large quantity of proteins. However, when proteins from other species such as mammalian are produced in this system, problems of protein ex...
Figure 1. Defining the canonical red blood cell (RBC) proteome with high accuracy from a synthesis of protein mass spectrometry and mRNA expression data (A) Measured protein and RNA abundances from diverse blood cell types and plasma were used as features for a machine learning classifier to ass...
Overexpression of each of the four target proteins was achieved using cDNA expression constructs, as assessed by western blot (Fig. 3a,b). As previously reported34, there is some cross-reactivity between GABARAP and GBRL1 antibodies. Additionally, in the case of the EGFP labelled GABARAP ...
Sph1 has been detected in Lewy bodies (LBs) associated with different a-synucleinopathies such as Parkinson's disease, diffuse Lewy body disease and multiple system atrophy8,9. Expression of Sph1 is also increased in the cortices of patients suffering from dementia with LB, Parkinson's disease...
The black arrows indicate the position of periplasmic inclusion bodies. Full size image Compartmentalized cellular stress responses induced by malE31 mutants To examine the alternative and competitive export and folding processes in the cytoplasm, we analyzed the cellular fates of MalE31 carrying either ...
10.1073/pnas.93.3.1314. Article PubMed PubMed Central Google Scholar Querfurth HW, Suhara T, Rosen KM, McPhie DL, Fujio Y, Tejada G, Neve RL, Adelman LS, Walsh K: Beta-amyloid peptide expression is sufficient for myotube death: implications for human inclusion body myopathy. Mol Cell ...
A mutant form of the ataxin-1 protein with an expanded polyglutamine (polyQ) tract is the underlying cause of the inherited neurodegenerative disease spinocerebellar ataxia 1 (SCA1). In probing the biophysical features of the nuclear bodies (NBs) formed
GFP1-10 detector protein is often produced in small scale shake flask cultivation and purified from inclusion bodies. Results The production of GFP1-10 in inclusion bodies and purification was comprehensively studied based on Escherichia coli as host. Cultivation in complex and defined medium as ...