protein foldingrecombinant protein expressionconformational disease modeldrug delivery systemsnanoparticulesThe formation of inclusion bodies (IBs) constitute a frequent event during the production of heterologous proteins in bacterial hosts. Although the mechanisms leading to their formation are not completely ...
Recombinant protein expression in bacteria often results in the formation of both inactive and insoluble protein that accumulates as intracellularprotein aggregates called inclusion bodies [1]. It has beenshown that 70-80% of recombinant proteins expressed in E.coli are as inclusion bodies [2]. Thi...
We have studied the structure and characteristics of inclusion bodies formed by the enzyme 尾-lactamase in the periplasmic space ofor in the cytoplasm, following expression of the protein without its signal sequence. Electron microscopy of highly purified protein aggregates using a novel sucrose ...
Metal (e.g., lead, mercury); viral particles (e.g., herpes, CMV); metabolically inactive materials (e.g., ceroid, Mallory bodies). Segen's Medical Dictionary. © 2012 Farlex, Inc. All rights reserved. inclusion body a body present in the nuclei or cytoplasm of cells infected by viru...
The formation of inclusion bodies (IBs) constitute a frequent event during the production of heterologous proteins in bacterial hosts. Although the mechanisms leading to their formation are not completely understood, empirical data have been exploited trying to predict the aggregation propensity of specifi...
The quality of the resulting protein can be assessed by SDS-PAGE. 展开 关键词: Inclusion bodies Recombinant protein expression Induction Escherichia coli Feedback inhibition Selenomethionine labeling Cell lysis Solubilization Protein concentration Aggregation ...
1988. Formation of recombinant protein inclusion bodies in E. coli. Trends in Biotechnol. 6:95–101. Article CAS Google Scholar Pigiet, V.P. and Schuster, B.J. 1986. Thioredoxin-catalyzed refolding of disulphide-containing proteins. Proc. Natl. Acad. Sci. USA 83:7643–7647. Article CAS...
High level expression of recombinant proteins produced in bacteria, such asE. coli,often results in formation of insoluble aggregates within the bacterial cell, known as inclusion bodies (Shein et al., Bio/Technology 7:1141-49, 1989). An inclusion body protein is one that is, in general, ov...
The formation of inclusion bodies (IBs) constitute a frequent event during the production of heterologous proteins in bacterial hosts. Although the mechanisms leading to their formation are not completely understood, empirical data have been exploited tr
Protein foldingRecombinant protein expressionEscherichia coliIncreasing evidence indicates that protein aggregation in bacteria does not necessarily imply loss of biological activity. Here, we have investigated the effect of growth-temperature on both the activity and stability of the inclusion bodies formed ...