One of the challenges in the study of EVs and there utility as diagnostic biomarkers is the amount of EVs needed for traditional protein analysis methods. Here, we present a new immuno-PCR method that takes advantage of commercially available TotalSeq antibodies containing DNA conjugated oligos to...
CAPRI The Critical Assessment of PRediction of Interactions (CAPRI) is a community-wide experiment to evaluate protein-protein interaction prediction methods PIFACE PIFACE is a web server for the prediction of protein-protein interactions. It identifies potential interaction interfaces on protein surfaces...
SarahZerbs, ...Frank R.Collart, inMethods in Enzymology, 2009 9.1Analysis of protein expression and solubility Validation ofprotein expressiontypically involves an assessment of expression and solubility of the target protein and a qualitative verification of the expected protein size. Most of theE....
To test the validity of this principle in a different expression chassis and construct library, we repeated the analysis in Fig.5using a recent genotype-phenotype screen of promoter sequences inSaccharomyces cerevisiae25. These data are comparable to the screen in Cambray et al.23in the sequence...
Alongside a systematic classification and discussion of relevant databases, feature extraction strategies, and prominent computational approaches, we conclude with a thorough analysis of current challenges and prospects for the future of this field. Keywords: protein–protein interactions; computational methods...
Genomic analysis Pat SLee,Kelvin HLee, inCurrent Opinion in Biotechnology, 2000 Despite the utility of these methods for investigating gene expression, studies in yeast[2••]and human liver cells[1]have shown thatprotein expressionlevels are not well-correlated withmRNA expression levels. Fur...
Identification and Expression Analysis of a Full-length cDNA Encoding a Kandelia candel Tonoplast Intrinsic Protein红树植物秋茄中液泡膜内在蛋白(TIP)全长cDN... Soil salinity is an important issue, as most crop plants are low in salt tolerance. Salt tolerance, a complex, multifactorial, and multigeni...
methods, making it simple to analyze biophysical properties, predict secondary structures such asB-cell epitopes, calculate surface probability, perform amino acid composition analysis, and more. What’s more, Protean 3D is a complete protein sequence analysis software package, meaning that after you ...
This invention provides methods for cytoplasmic detection of protein-protein interactions, nuclear export/localization sequences, and galactose-independent inducible Gal4p-mediated gene expression through the utilization of GAL regulatory factor, Gal80p, and the yeast galactose regulon. A cDNA encoding a ...
Expression and binding specificity analysis of odorant binding protein AlucOBP7 from Apolygus lucorum (Hemiptera: Miridae). Acta Entomologica Sinica, 2013, 56... 纪萍,刘靖涛,谷少华,... - 《昆虫学报》 被引量: 23发表: 2013年 Molecular Characterization and Expression Profiles of Polygalacturonase ...