In this study, the protein content in a laboratory GMP preparation and two commercial GMP samples, which was estimated by the Bradford, biuret, Lowry and bicinchoninic acid (BCA) assays, were compared using the Kjeldahl method. There were apparent differences in the values of estim...
Protein estimation in the lysate supernatant was done using a bicinchoninic acid (BCA) method, a concentration (1 mg/ml) of protein lysates was precleared with 40 μl of protein A/G beads for 1 h, the supernatant was incubated for 1 h at 4 °C in HTT IgG (MAB2166) or ...
Protein concentration was determined using the BCA method (Thermo, Pierce). Quantification of protein levels Quantitative Western blotting was performed using ImageJ and Image Studio Lite (LI-COR). Protein levels were normalized to the total protein amount in the sample visualized by a Coomassie ...
12Estimation of Protein Concentrations in Samples Once samples have been isolated and are ready for treatment, it will be important to carry out aprotein assay, as samples will need to be compared, either as different lanes on the same gel or across different gels. ...
Fig. 3. Estimation of protein mass per cell using two biochemical approaches and the proteomic ruler method. A, the histone proteomic ruler concept. The mass of cellular DNA is approximately equal to the protein mass of histones. Relating the histone MS signal to the total MS signal therefore...
A scalable approach for protein false discovery rate estimation in large proteomic data sets. Mol. Cell. Proteom. 14, 2394–2404 (2015). Article CAS Google Scholar Gassaway, B. M. et al. A multi-purpose, regenerable, proteome-scale, human phosphoserine resource for phosphoproteomics. Nat. ...
Pierce™ Dilution-Free™ Rapid Gold BCA Protein Assay, 500 mL Catalog number: A55860 2,179.00/Each Quant-iT™ 蛋白检测试剂盒 Catalog number: Q33210 2,307.00/Each Pierce™去污剂兼容型 Bradford 检测试剂盒 Catalog number: 23246 2,246.00/Each ...
concentrationsfor substancestestedwiththeTestTubeprocedureonthemidpoint concentrationofBSAstandard(i.e.,1000µg/mLformost assays;10µg/mLfortheMicroBCAAssay).Substanceswere consideredcompatibleattheindicatedconcentrationiftheerror inproteinconcentrationestimationcausedbythepresenceof thesubstanceinthesamplewas...
Folin-Ciocalteau phenol reagentbicinchoninic acidBCAmicrowaveprotein assayWe present here microwave-based modifications of standard protein assays that dramatically ... RE Akins,RS Tuan - 《Molecular Biotechnology》 被引量: 28发表: 1995年 Protein estimation with the Folin Ciocalteau reagent Folin-Ciocalteau...
Percolator was used for false discovery rate (FDR) estimation and only rank 1 peptide identifications of high confidence (FDR <1%) were accepted. TMT reporter values were assessed through Proteome Discoverer v2.3 using the Most Confident Centroid method for peak integration and integration tolerance ...