than the dsGFP-treated control. Electrical penetration graph (EPG) was adopted for monitoring the insect feeding behavior. Compared with dsGFPtreatment,L. striatellustreated with dsLsSP1exhibited a significant decrease (by 62%; two-tailed unpaired Student’sttest,p = 0.0057) in phloem sap i...
Here, we pursued this analysis for blood pressure (BP) regulation.Methods The relational scheme of the implemented in Microsoft SQL Server BP-GWAS meta-database enabled the combined storage of: GWAS data and attributes mined from GWAS Catalog and the literature, Ensembl-defined SNP-transcript ...
The blood was allowed to stratify at room temperature for 2 h before centrifuging at 3,000g for 10 min to remove precipitates. The supernatants (100 µL) were then added to 400 µL of ice-cold acetone and allowed to stratify at −40 °C for 30 min before centrifuging at 10,...
Blood. 2014;124(21):5467. http://www.bloodjournal.org/content/124/21/5467?sso-checked=true. Poddubnaya I, Babicheva L, Kaplanov K, Zaritskey A, Volodicheva E, Alexeev S, et al. Comparison of pharmacokinetics and pharmarodynamics of BCD-020 with innovator rituximab to palients with ...
Collagenase stimulatory factor; EMMPRIN; Extracellular matrix metalloproteinase inducer; Leukocyte activation antigen M6; M 6; M6; M6 leukocyte activation antigen; Neurothelin; OK; OK blood group; OK blood group antigen; TCSF; Tumor cell derived collagenase stimulatory factor; Tumor cell-derived collag...
coatneyi blood stage parasites (Fig. 1D), providing additional evidence that PvRMC-MSP1 induces antibodies that are capable of recognizing the naturally derived MSP-1 protein. The PvRMC-MSP1 T cell epitopes can act as universal epitopes given their structural features, including a large ...
Aortas were flushed with ice-cold M199 medium until free of blood (n=3 from each group). The adventitia was dissected free, and the aorta was cut into multiple 1-mm rings under a dissecting microscope. 24-well culture plates were coated with 400 μL of growth factor-reduced Matrigel (BD...
thus facilitating blood-feeding and viral pathogen transmission3,4. Saliva proteins secreted byAedes aegypti, such as CLIPA3 protease and a 34-kDa protein, can enhance Dengue virus (DENV) replication and suppress host innate immune responses5,6. Similarly, a saliva protein fromA. aegypti, LTRIN...
These proteins exhibit a potent lytic activity on human erythrocytes and peripheral blood mononuclear cells. Like SjSP-13, SjSAPLP4, and SjSAPLP5 are candidate diagnostic markers for schistosomiasis japonica [16]. SjSP-13 is highly expressed in young worms and adult worms but poorly expressed ...
Filter blood through cheesecloth and keep on ice, then centrifuge at 2000 RPM for 10 min. When cell density reaches 4 × 106 cell/mL, centrifuge culture at 200× g for 10 min. Wash once with buffer (40 mM HEPES KOH, pH 7.5, 100 mM KOAc, 4 mM DTT). Harvest when cell density ...