(pH 8.6) so that most protein will have a negative charge. Molecules with a negative charge move toward the anode (positive electrode) when anelectric currentis passed through the solution. Aside effectofagaroseelectrophoresis is that electroen-dosmosis occurs because of impurities in the agarose,...
It was not observed significative correlation (P>0.05), according to the Spearman non-parametric test, either between the Strongyloides eggs count per gram of feces or the Haemonchus spp. larval count per gram of feces and the fraction electrophorectly variable. 展开 关键词:...
The mixture was electroporated into HeLa cells using 4D-Nucleofector (Lonza). Cells were recovered by DMEM with 10% FBS and cultured on a 35-mm dish for 24 h. Single-cell clones were isolated using limiting dilution in 96-well plates. To edit the XPA gene, a guide RNA (gRNA) ...
Bcl-xL (4.6 × 4.6 × 3.5 nm), FKBP12 (4.5 × 3.5 × 3.0 nm), and MDM2 (3.0 × 3.7 × 3.8 nm). To test whether the proteins are still folded in the nanopore condition, we further performed
(ThermoFisher). Secretion of viral RNA was assessed by amplifying the structural genes using the High-fidelity Phusion polymerase (ThermoFisher) with gene-specific primers. Amplicons were resolved by 1% agarose gel electrophoreses and recorded using the GelDoc software (INTAS). Primers used for ...
For blood-derived cells, human PBMCs from one healthy donor were prepared using the lymphocyte separation medium (Eurobio, Les Ulis, France) as described by the manufacturer. One million PBMCs were plated in a 12-well dish and cultivated 1 hour at 37 °C in DMEM. The adherent fraction (...
identify wound healing stages36as it is less likely to be affected by the active external environment surrounding the exudate. Albumin was the protein determined in this research work, which has been modelled and measured previously37,38as it is the most abundant protein in blood plasma (it ...
The sample plate is placed directly on the electro optic detection crystal such that the aperture is over the near infrared (NIR) probe incident on the electro optic crystal from the bottom surface. The electro optic crystal also acts as the bottom of an environmental chamber for the sample. ...
The parasite is transmitted during the vector blood meal on an infected vertebrate with the ingestion of infected cells or amastigote forms that have reached the extracellular milieu in the mammalian host. In the vector midgut, the protozoa are enclosed in the peritrophic matrix, in contact with ...
Compared with the peripheral blood test results of healthy controls, the expression of circulating CST1 in patients with gastric cancer was increased and was closely related to disease progression. Furthermore, ROC curve analysis indicated that CST1 improved the sensitivity and specificity of the ...