Wearable electrodriven switch actively delivers macromolecular drugs to fundus in non-invasive and controllable manners Current treatments for fundus disorders face safety and efficacy challenges. Here, the authors developed a wearable electrodriven switch that enables macromolecule delivery to the fundus ...
The mixture was electroporated into HeLa cells using 4D-Nucleofector (Lonza). Cells were recovered by DMEM with 10% FBS and cultured on a 35-mm dish for 24 h. Single-cell clones were isolated using limiting dilution in 96-well plates. To edit the XPA gene, a guide RNA (gRNA) ...
(pH 8.6) so that most protein will have a negative charge. Molecules with a negative charge move toward the anode (positive electrode) when anelectric currentis passed through the solution. Aside effectofagaroseelectrophoresis is that electroen-dosmosis occurs because of impurities in the agarose,...
Blood exposure to artificial surfaces, such as dialysis membranes, leads to the deposition of a plasma protein layer over the polymer and within the membrane pores. Protein adsorption is a complex phenomenon governed by hydrophobic and electrostatic interactions, hydrogen bonding and Van der Waals forc...
ElectrochmiluminescenceHydrogen sulfideSmall molecule biomarkerType 1 diabetes2023 The AuthorsHydrogen sulfide (H2S), an antioxidant and cell signaling molecule, plays a critical role in redox reactions in the biological system, and its imbalances in blood levels have been implicated in various diseases...
poly- merase chain reaction; PDIs: protein disulfide isomerases; PVDF: polyvinylidene difluoride; RT: room temperature; RT-qPCR: quantitative reverse transcription PCR; SDS: sodium dodecyl sulfate; SDS-PAGE: polyacrilamide gel electropho- resis with SDS; SNP: single nucleotide polymorphism; UPR: un...
Fig. 10.Monoclonal peak quantitation. Serum protein CE electropheretograms (Sebia Capillarys) was performed. Quantitation by: (A) standard perpendicular drop method (shaded) where the demarcation begins where the monoclonal protein meets the polyclonal region below it (arrows) and then proceeds straig...
In blood plasma, lipoproteins carry insoluble lipids in the aqueous medium. Metalloproteins represent a large group of proteins that are conjugated to metals (Fe, Cu, Zn, Mg, Mn) which are essential for the structure and function of those molecules. View chapter Book 2022, Medical Biochemistry...
(HBV) shed excess viralsurface proteininto the blood; that is,hepatitis B surface antigen(HBsAg). HBsAg was identified as a 22-nmlipoproteinparticle antigen with protective epitopes. To develop a vaccine, plasma was harvested from long-termchronic hepatitis Bcarriers, HBsAg purified, and the ...
Here, we describe a fundamental and isovolumetric process whereby electroneutral ion transport buffers intracellular osmotic potential against daily and acute variations in cytosolic soluble protein abundance. Consequently, the ionic composition of the cell is not constant throughout the day, as has been...