What this study adds: The study adds to the evidence on SFLC test utilisation. Serum protein electrophoresis alone may miss cases of light chain myeloma, while SFLC performed in isolation may produce false positive results in the setting of inflammatory disorders or renal impairmen...
Fig. 10.Monoclonal peak quantitation. Serum protein CE electropheretograms (Sebia Capillarys) was performed. Quantitation by: (A) standard perpendicular drop method (shaded) where the demarcation begins where the monoclonal protein meets the polyclonal region below it (arrows) and then proceeds straig...
Proteinelectrophoresisis a widely used screening test formonoclonal gammopathies. Again, multiple methodologies are in practice. This chapter will deal withserum proteinelectrophoresis by conventional gel methods as well ascapillary electrophoresis. Urine electrophoresis, CSF electrophoresis, as well asimmuno...
To test the suitability of the BGEs, initial experiments used serum samples (7 mg/mL) pre-incubated for 30 min with 38 nM fluorescein and excess monoclonal antibody (334 nM). Figure 3 shows fluorescence electropherograms for pre-incubated serum samples in the three buffer systems previously ...
Serum level of insulin was measured by the electrochemiluminescence immunoassay method. HOMA used indices of pancreatic -cell function and tissue insulin sensitivity. Differences between groups were compared by one-way analyses of variance and the post hoc TukeyHSD test for multiple comparisons; however...
For electroporation, 100–150 embryos (1 h after thawing) were placed into a chamber with 40 μl of serum-free medium (Opti-MEM, Thermo Fisher Scientific) containing 100 ng μl−1 Cas9 Nuclease V3 and 100 ng μl−1 Csb gRNA. They were then electroporated with a 5-...
In this research, we designed an ultrasensitive ECL immunosensing framework for determination of HER-2 protein biomarker in serum samples. In this regard, [Ru(bpy)3]2+/TPrA was used as anodic ECL system. We employed electrodeposition method for uniform layer of rGO on the electrode. Using CS...
they are not produced by the cancer cells themselves, but by neutrophils recruited to the site of the tumour. The detection in the blood of S100A8/S100A9 released by neutrophils in the tumour microenvironment suggests they could be utilised as a serum biomarker for diagnosis or prognosis of CA...
Serum inflammatory markers were increased in COVID-19 cases with severity. M−band(s), β-γ bridging and pre-albumin band(s) on SPEP were observed in 15.5, 11 & 12% of severe cases and 3, 4 & 0% moderate COVID-19 cases respectively. Area under curve (AUC) of α 1 and α 2...
Vitamin D deficiency (serum 25(OH) D < 50nmol/L) was detected in all subjects (Table 1) as measured using electrochemiluminescence immunoassay on Roche Elecsys Cobas e411 analyzer (Roche Diagnostics, GmbH, Mannheim, Germany). Serum concentration of total cholesterol (CHOL), high density lip...