Also Known As: Serum Protein Electrophoresis, Protein ELP, SPE, SPEP, Gel Electrophoresis, Capillary Electrophoresis, Immunosubtraction Electrophoresis, Urine Protein Electrophoresis, UPE, UPEP, IFE, CSF Protein Electrophoresis, Electrophoresis Board Approved At a Glance Why Get Tested? To help diagnos...
HUMAN SERUM FOLATE CAN BE MEASURED USING FOLATE BINDING PROTEIN LINKED TO ENZYME-LABELED PROTEIN LIGAND BINDING ASSAY (ELPLBA) AS WELL AS ELISAdoi:10.32889/ACTABIOINA.V1I2.17Muhamad Arif BudimanMohamad SadikinAni Retno PrijantiIndonesian Society for Biochemistry and Molecular Biology...
Serum protein electrophoresis (SPE) separates serum proteins into bands whose shape and amplitude can alert clinicians to a range of disorders. This is followed by more specific immunoassays to quantify important antigens and confirm a diagnosis. Here we develop a high-speed capillary electrophoresis ...
Initially, the NIH/3T3 cells were cultured in serum-free medium containing different concentrations of EEP, EP, and rhEGF standard. After 24 h, it was found that the cell viability of each group increased, especially at a concentration of 50 nmol∙L−1 (Fig. 3(a)). There was no ...
Cell culture media (Dulbecco's Modified Eagle Medium, DMEM, and Roswell Park Memorial Institute, RPMI) and supplements (heat inactivated Fetal Bovine Serum, FBS, and non-essential amino acids, NEAA) were purchased from Invitrogen (Gibco, Invitrogen Corporation, Paisley, UK...
Cells were grown in Dulbecco's modified Eagle's medium (DMEM) or RPMI medium supplemented with 10% fetal bovine serum (FBS), 1% (v/v) non-essential amino acids, 1% sodium pyruvate, and 1% penicillin/streptomycin/glutamine (Sigma-Aldrich, St. Louis, MO). The cells were grown at 37 ...
(i.e. a phosphorylation site enumerated in Column E of Table 1, which comprises the corresponding phosphorylatable amino acid listed in Column D of Table 1), collecting immune serum from the animal, and separating the polyclonal antibodies from the immune serum, in accordance with known ...
Medium then was replaced with F-12K containing 8.5% fetal bovine serum and cells were treated with ethanol (100 mmol/L, 459836; Sigma-Aldrich) for 48 hours. Treated plates were enclosed in a MIC-101 (Billups-Rothenberg; http://www.brincubator.com/hypoxiachamber.htm) hypoxia chamber together...
1. Introduction Albumin is the most abundant serum protein with concentration of about 35–50 mg/mL [1]. It is a cargo protein for a diverse array of endogenous and exogenous small molecules [2] and it has several very high affinity binding sites for fatty acids [3]. Albumin is a ...
Serum levels of soluble programmed cell death protein 1 and soluble programmed cell death protein ligand 2 are increased in systemic lupus erythematosus and associated with the disease activity. Lupus 2020, 29, 686–696. [CrossRef] 36. Canavan, M.; Floudas, A.; Veale, D.J.; Fearon, U....