Serum protein electrophoresis (SPE) separates serum proteins into bands whose shape and amplitude can alert clinicians to a range of disorders. This is followed by more specific immunoassays to quantify important antigens and confirm a diagnosis. Here we develop a high-speed capillary electrophoresis ...
HUMAN SERUM FOLATE CAN BE MEASURED USING FOLATE BINDING PROTEIN LINKED TO ENZYME-LABELED PROTEIN LIGAND BINDING ASSAY (ELPLBA) AS WELL AS ELISAdoi:10.32889/ACTABIOINA.V1I2.17Muhamad Arif BudimanMohamad SadikinAni Retno PrijantiIndonesian Society for Biochemistry and Molecular Biology...
Initially, the NIH/3T3 cells were cultured in serum-free medium containing different concentrations of EEP, EP, and rhEGF standard. After 24 h, it was found that the cell viability of each group increased, especially at a concentration of 50 nmol∙L−1 (Fig. 3(a)). There was no ...
Evans blue (EB), an aromatic dye with four anionic charges, reversibly binds to serum albumin with a micromolar affinity. The affinity of EB for albumin allows 100% of the injected dose to be retained in the blood; thus, it can be used to quantify total plasma volume of a test subject...
Cells were grown in Dulbecco's modified Eagle's medium (DMEM) or RPMI medium supplemented with 10% fetal bovine serum (FBS), 1% (v/v) non-essential amino acids, 1% sodium pyruvate, and 1% penicillin/streptomycin/glutamine (Sigma-Aldrich, St. Louis, MO). The cells were grown at 37 ...
In the present study, a lab-based consumer acceptance test was conducted to obtain a general perspective on how consumers accept the products. To evaluate consumer acceptance of protein-enriched foods or meals in real life, contextual aspects could be considered and included for exploration in ...
One-way ANOVA parametric test was also applied to compare the mean values of all Wild Type cell lines to the mean of all DMD cell lines results (* p < 0.05; ** p < 0.01; *** p < 0.001). Supplementary Materials: Supplementary materials can be found at www.mdpi.com/1422-0067/19/...
Herein, we aim to test the hypothesis that FKBP8 may regulate CLC-1 protein at the plasma membrane. Surface biotinylation and subcellular fractionation analyses reveal that a portion of FKBP8 is present at the plasma membrane, and that co-expression with CLC-1 enhances surface localization of ...
Bovine serum albumin (0–100 ppm) was used to prepare a standard curve for quantifying the protein contents of the extracts. Based on the protein content of the extract, the protein extraction yield (g/100 g sample) was calculated using following formula: Extraction yield (%) = (Protein ...
3.6. Bovine Serum Albumin Binding Studies 3.6.1. UV-Vis Spectra The BSA stock solution was prepared in 50 mM phosphate buffer saline (PBS)/100 mM NaCl buffer at pH 7.4 to attain a final concentration of 1 µM, and stored at 4 ◦C with constant ionic strength. The PBS buffer (3 ...