Coding nucleotide sequence of MG53 (mitsugumin53) protein with codon optimization, recombinant of MG 53 protein as well as application of MG 53 protein本发明涉及一种密码子优化的MG53蛋白的编码核苷酸序列,其重组体及其应用;本发明属于基因工程领域/生物医药技术领域. The present invention relates to a ...
The density of protein-coding sequence variation in ExAC reveals a number of properties of human genetic variation that are undetectable in smaller data sets. For example, 7.9% of high-quality sites in ExAC are multiallelic (multiple different sequence variants observed at the same site), close ...
coding the PDK1 of the cashmere goats is designed; and the pair of primers is used to amplify a specific fragment through an RT-PCR method, and after sequencing, 698bp nucleotide sequence and the amino acid sequence corresponding to the nucleotide sequence from the 2nd to the 697th locus ...
A protein-coding gene is composed of a series of nucleotide triplets – the codons – that encrypt not only the protein content but also the start and stop signals. There are 64 (43) codons in the canonical genetic code, which encode 20 amino acids with redundancy. Hence, there are synony...
Fourth, adding non-protein modalities (e.g. non-coding regulatory elements) as input to gLM may also greatly improve gLM’s representation of biological sequence data, and can learn protein function and regulation conditioned upon other modalities51. Finally, our model was trained largely on ...
Left, the coding sequence of the gene of interest is amplified and cloned into a plasmid under control of an SP6, T3, or T7 promoter. The linearized plasmid DNA can be used for in vitro transcription. Circular plasmid DNA can also be used for in vitro translation using the TNT Quick ...
MICROORGANISMS AS CARRIERS OF NUCLEOTIDE SEQUENCES CODING FOR ANTIGENS AND PROTEIN TOXINS, PROCESS OF MANUFACTURING AND USES THEREOF The invention relates to a microorganism as a carrier of nucleotide sequences coding for antigens and protein toxins comprising the following components: (... I Gentsch...
Exploiting sequence–structure–function relationships in biotechnology requires improved methods for aligning proteins that have low sequence similarity to previously annotated proteins. We develop two deep learning methods to address this gap, TM-Vec and DeepBLAST. TM-Vec allows searching for structure–...
While protein language models have so far focused on amino acid sequences, there is additional information contained in the DNA sequence encoding the protein. The language of protein-coding DNA (cDNA) relies on 64 nucleotide triads, known as codons, each of which encodes a specific amino acid ...
(a). Viral RNA contained therein was reverse transcribed and an ~1,000 nucleotides long region within the structural protein coding sequence was amplified by PCR. The non-replicative NS5 mutant (GAA) served as negative control (lane 3). DNA size maker is shown in lane 1 (n = 2)....