Plant cells fully control and initiate their aging and death during thelife cycle. They use theirprotein degradationsystems to maintainproteostasisand transport nutrients from aging organs.Protein cleavageis an important catabolic process, it includes many proteases, regulators, varioussubcellular localizations...
Virus-derived P2A peptide induces protein cleavage during translation21,22,23. Cleaved proteins should be synthesized at the same molecular numbers, because they are translated by the same ribosome. Therefore, by placing GFP at the C-terminal of P2A peptide, the expression level of the target ...
Cx is a simple measure of the protrusion degree of protein atoms, related to the atomic environment, that can be used to characterise binding sites, cleavage sites, etc [59]. ASA [60] is a shape descriptor that has been extensively employed in protein structural analysis to describe, amongst...
Native test case proteins and FXN TIM designs were produced as fusions to an N-terminal 6xHis tag followed by a Tobacco Etch Virus (TEV) cleavage sequence (ENLYFQS). FXC TIM designs were produced as fusions to a C-terminal 6xHis tag. Expression was performed in E. coli BL21(DE3) usin...
V. In silico screening and testing of FDA approved small molecules to block SARS-CoV-2 entry to the host cell by inhibiting spike protein cleavage. Viruses 14(6), 1129. https://doi.org/10.1101/2022.03.07.483324 (2022). Article CAS PubMed PubMed Central Google Scholar Kulkarni, S. A. ...
4. Newton K, Wickliffe KE, Dugger DL, Maltzman A, Roose-Girma M, Dohse M, Kőműves L, Webster JD, Dixit VM (2019) Cleavage of RIPK1 by caspase-8 is crucial for limiting apoptosis and necroptosis.Nature574:428–431. 5. Li Y, Zhong CQ, Xu X, Cai S, Wu X, Zhang Y, Chen...
Perturbing La expression, cleavage or surface function inhibits osteoclast fusion, while exogenous, surface La promotes fusion. Moreover, the mechanism by which La promotes osteoclast fusion is independent of La’s ability to interact with RNA through its highly conserved La domain. Indeed, a C-...
Ion mobility (IM) mass spectrometry provides structural information about protein shape and size in the form of an orientationally-averaged collision cross-section (CCSIM). While IM data have been used with various computational methods, they have not yet been utilized to predict monomeric protein ...
(CPN1) gene, a metalloprotease that regulates other proteins through specific cleavage of amino acids from the C terminal. CPN1 has been shown to be a pleiotropic regulator of inflammation51. Expression for CPN1 was not available in our data. However, a strong association signal was identified...
7d). To assess the formation of RNAPII DPCs directly, we performed Cleavage Under Targets and Tagmentation (CUT&Tag) by using antibodies against RPB1 CTD-pS5 or CTD-pS2 under high salt conditions and without fixation to enrich for cross-linked RNAPII. We observed substantial formaldehyde-induced...