THE DIALYSIS OF TRYPSIN AND THE PROTEOCLASTIC ACTION OF THE PROTEIN CLEAVAGE PRODUCTSPeptidesProteinsData Interpretation, StatisticalNuclear Magnetic Resonance, BiomolecularDue to the inherent complexity of the natural biological environment, most studies on polypeptides, proteins and nucleic acids have so ...
The authors will be happy to provide the cleavage site-specific antibodies directed against shed PrP and characterized herein as research tool upon reasonable request.Abbreviations aa: Amino acid Ab: Antibody Aβ: Amyloid-beta peptide AD: Alzheimer’s disease ADAM: A disintegrin and ...
Labeled proteins were purified by dialysis (Slide-A-Lyzer MINI 10 K; Thermo Fisher Scientific) overnight, and the degree of protein labeling was determined using a NanoDrop spectrophotometer. MPPD screening protocol for RB-specific aptamers During each round of MPPD, ~108 aptamer particles (the...
For in situ protease cleavage, the plasmids based on the pMCSG19 vector were introduced into the E. coli BL21(DE3) competent cells containing the pRK1037 vector. The single colony was cultured on 2xYT plates containing 100 μg/mL ampicillin and 50 μg/mL kanamycin, and then inoculated...
After dialysis protein was aliquoted and stored at −80 °C until further use. Expression and purification of EF-P A pBAD-His6-SUMO plasmid harboring the efp gene was used to transform chemically competent E. coli TOP10 cells (standard heat-shock protocol) and plated on LB-agar plates...
(v1.6.3.4)71with the automatic setting for tryptic peptide matching and label free analysis, according to the following settings: protein sequence database: Swissprot human; protease: trypsin; cleavage site: lysine and arginine; number of missed cleavages: 2; fixed and variable modifications: ...
Besides the binding of the R-loop to the SP domain, there are additional interaction sites to enable the specific binding and cleavage. The carboxyl-terminus of plasma C4 α-chain, which consists of 11 negatively charged residues including three sulfotyrosines, interacts electrostatically in long...
(pH 6.0 or 8.0); ATRP-initiator immobilization on the agarose-supported protein (using N-2-bromo-2-methylpropanoyl-β-alanine N′-oxysuccinimide bromide (NHS-Br) as previously described17); surface-initiated ATRP to grow polymers from agarose-supported proteins15; and, cleavage of the resulting...
Liquid-liquid phase separation (LLPS) has emerged as a crucial biological phenomenon underlying the sequestration of macromolecules (such as proteins and nucleic acids) into membraneless organelles in cells. Unstructured and intrinsically disordered doma
(Fig.1b). A decrease in the hydrophobicity of these compounds, through the enzymatic cleavage of theN-terminal moiety (and spontaneous 1,6-elimination in the case ofAPmoc-F(CF3)F), is expected to lead to the destabilization of the supramolecular nanofibers and subsequent collapse of the ...