183,184]. These studies have shown that tunable and reversible phase behaviors can, to some degree, be encoded into de novo synthetic proteins by combining low complexity domains in particular arrangements [183]. Using this knowledge, researchers...
Proteins were detected by immunoblot using anti-GST (Santa Cruz Biotechnology, Santa Cruz, CA, USA) and anti-His (Sigma-Aldrich, St. Louis, MO, USA) antibodies. Phylogenetic Analysis RPP7 protein sequences were identified from an NLR Ren-seq data set (Van de Weyer et al., 2019) (http...
To determine whether the GSEA results reflected an actual increase in ribosomes, we began by assessing the 80 distinct ribosomal proteins (RPs) associated with eukaryotic ribosomes27. Although recent work has discovered that ribosomes can exhibit a specialized composition of RPs, the majority of rib...
The rapid evolution of viruses generates proteins that are essential for infectivity and replication but with unknown functions, due to extreme sequence divergence1. Here, using a database of 67,715 newly predicted protein structures from 4,463 eukaryoti
The use of diverse ligands and proteins proves experimentally that a high degree of control over biomolecular recognition has been established computationally. The biological and biosensing activities of the designed receptors illustrate some of the potential applications of computational design. The ...
using the combination of both. Additionally, mice were vaccinated with PcrV, OprF, or even the mix of these two proteins. Immunization with either mRNA-PcrV or mRNA-OprF-I elicited a Th1/Th2 blended or slighted Th1-biased immune reaction, conferred wide defense, and decreased microbial ...
(Y3). These factors had been subsequently optimized using a central composite rotatable design. Optimal values of feedback factors had been determined using the way of the energy purpose. The greatest energy worth of 0.88 ended up being obtained by the following optimal group of conditions waste ...
Therefore, these three CFPS systems have greater potentials for the design and production of future drug proteins. However, due to the relatively low levels of protein synthesis in B. subtilis, C. glutamate and V. natriegens systems compared to E. coli system, some applicatio...
De novo-designed translation-repressing riboregulators for multi-input cellular logic Article 04 November 2019 Spatiotemporal control of RNA metabolism and CRISPR–Cas functions using engineered photoswitchable RNA-binding proteins Article 30 November 2023 Data availability All data supporting the findin...
De novo engineering of intracellular condensates using artificial disordered proteins. Nat. Chem. 12, 814–825 (2020). Article CAS PubMed PubMed Central Google Scholar Chen, D. et al. Integration of light and temperature sensing by liquid–liquid phase separation of phytochrome B. Mol. Cell ...