(C) The structure of a complex of U1A and RNA ***(PDB ID: 1URN). 4. Discussion Many machine learning methods have been employed to predict the interactions between proteins and nucleic acids. However, most of the learning methods treat the problem of nucleic acid–protein interactions as ...
Once amplified and verified by sequencing, the gene can be introduced into a plasmid designed for protein expression. Many plasmids have been optimized for protein expression and contain promoter regions that allow expression to be induced at a designated time. The expression vector containing the ...
Site-directed mutagenesis was performed on the His9-SARS-CoV-2 Nucleocapsid pGEX vector to create the N protein constructs (SI Table S1) and sequences were verified using Sanger sequencing. All variants were expressed recombinantly in BL21 Codon-plus pRIL cells (Agilent) or Gold BL21(DE3) ...
A. et al. Optimization of affinity, specificity and function of designed influenza inhibitors using deep sequencing. Nat. Biotechnol. 30, 543 (2012). Article CAS PubMed PubMed Central Google Scholar Silva, D.-A. et al. De novo design of potent and selective mimics of il-2 and il-15....
Therefore, using whole-exome and whole-genome sequencing (WES and WGS, respectively), we aimed to identify novel PAVs and protein-truncating variants (PTVs, as putative loss-of-function variants) affecting serum lipid and lipoprotein measurements, complemented with serum nuclear magnetic resonance (...
Nucleic acid and/or protein macromolecule based nanomedicines present an exciting avenue in medical care. Within the last three decades almost three-thousand gene therapy clinical trials have been performed, and at least nine gene therapy products reached medical market authorization, see [1] and http...
Conversely, this information can be experimentally obtained through protein-binding microarray11, systematic evolution of ligands by exponential enrichment combined with high-throughput sequencing (SELEX–seq)12, chromatin immunoprecipitation followed by sequencing13, high-throughput SELEX14 or related high-...
The gel electrophoresis mobility shift assay (EMSA) is used to detect protein complexes with nucleic acids. It is the core technology underlying a wide range of qualitative and quantitative analyses for the characterization of interacting systems. In the
We have used this approach to comprehensively map RNA-binding protein interaction sites transcriptome-wide in both plants and animals (termed protein interaction profile sequencing, PIP-seq) (see Fig. 2, and our unpublished results). These approaches are an RNA-centric means to define RNA–protein...
为了分析蛋白质序列中是否存在语言学中的Zipf定律,从蛋白质二级结构数据库DSSP中抽取1。 更多例句>> 4) protein sequences 蛋白质序列 1. Classification of protein sequences based on frequent patterns; 基于频繁模式的蛋白质序列分类 2. Based on the completeness of genome sequencing projects of the Human...