protein species had been used earlier in the literature [3, 4], it was not clearly defined and used more in the sense of a protein complex consisting of several subunits [3] or to differentiate between different proteins (e.g. catalase and actin were two different protein species) [4]. ...
which combines the IgG-binding domains of both Protein A and Protein G. Protein A/G enables capture of immunoglobulins from a wider range of species and antibody isotypes than either Protein A or Protein G alone. The bea...
Silica nanoparticles activate defense responses by reducing reactive oxygen species under Ralstonia solanacearum infection in tomato plants. NanoImpact 28, 100418 (2022). Article CAS Google Scholar Keshishian, H. et al. Quantitative, multiplexed workflow for deep analysis of human blood plasma and ...
PLD Analysis of a, Pex13 b, Pex5 and c, Pex14 using PLAAC21. Predicted PLD sequence is marked in red for Pex13 and Pex5 on the left, corresponding to the outputs on the right. d, Multiple sequence alignment of the Pex13 sequence from the indicated five species, showing conserved Y ...
Low-affinity and instant protein-protein interactions may not be detected; the binding of the two proteins may not be a direct binding, but a third party may act as a bridge in the middle; the target protein must be predicted before the experiment to choose the final Antibodies tested, so...
Prediction of protein-ligand binding affinity with deep learning 1Introduction Proteins are responsible for a wide range of life activities in organisms, with up to three billion in human cells[1]. However, proteins cannot work alone in the body and must bind to other molecules, known as ligand...
The buried surface area of A. fumigatus alkaline protease 1 (3471.7 Å2) was greater than that of the furin protease (1796.7 Å2). This suggests that the binding affinity between A. fumigatus alkaline protease 1-SARS-CoV-2 spike protein was stronger, with a larger binding surface area ...
Family-B GPCRs have been found in all animal species investigated, including mammals, Caenorhabditis elegans and Drosophila melanogaster, but not in plants, fungi or prokaryotes. In this article, I describe the structures and functions of family-B GPCRs and propose a simplified nomenclature for ...
In the absence of such an RFP, biosensing is limited to utilizing multiple RFP species where inseparable cross-talk contaminates all FRET readouts involved. The TagRFP chromophore has a large 2-photon absorption cross-section at a region where its one-photon extinction is low33, making it a ...
processed to generate Aβ amyloid species17,18,19. However, recent work suggests that APP can be trafficked directly from the Golgi to EEA1-positive early endosomes via an AP-4-dependent process, and that this pathway may be the dominant trafficking route in some cell types20,21. There is...