Measuring protein concentration Accurately quantifying total protein concentration is a key step in most experiments and workflows involving isolation, separation, and analysis of proteins. We offer a variety of assay reagents, kits, and standards for protein quantitation by fluorescent or colorimetric dete...
- 惰性且稳定—出色的制造方法可通过耐浸出的共价键固定蛋白 A/G。 数据: Manual vs. automated purification methods Thermo Scientific™ Pierce™ Protein A/G Magnetic Agarose Beads are suited for purifying IgG by manual or automated applications. IgG was purified from mouse, human, and rabbit sera...
Thermo Scientific Pierce 蛋白 A/G 磁珠是一种高性能亲和微粒,可用于抗体纯化和免疫沉淀方法(使用手动或自动磁性分离器)。 蛋白 A/G 磁珠的特点: ? 高容量 — 结合容量几乎是其他供应商的典型磁珠的 4 倍,每次实验
Protein quantitation assays, immunoprecipitation (IP) beads and kits, western blotting supplies and equipment, ELISA kits, multiplex assays for the Invitrogen Luminex platform, enzyme reporter assays, and protein activity assays Protein Mass Spectrometry Analysis ...
Washing for at least 3 times 2 e r D u t e a n n a e t d u n r o e 3 N 4 磁条:可抽出 7. Elution Magnetic Beads 磁力架使用说明 antibody Sample Magnetic Separator 2. 磁珠预处理 5 1 Binding buffer Remove supernatant 将磁珠漩涡振荡1 min,使其充分混悬;取25~50 µL磁珠悬液...
(40150-R007, Sino Biological, China) were then added for other 0.5 h incubation, then mixed with 20 μL of protein A beads (10001D, ThermoFisher, USA) at 4 °C for 0.5 h with slow rotation to reduce non-specific binding. The immunoprecipitates were washed for five times ...
The beads are magnetically captured, electrically stimulated and the light emission is proportional to the binding of ligand. A common label is Ruthenium (II) tri-bipyridine, NHS ester. electrodynamic forces London-Van der Waals forces: see DLVO theory. electrofocusing Any technique whereby chemical...
Protein A/G Matrix免疫沉淀磁珠及试剂盒系列产品是利用百奥莱博纳米表面技术使Protein A/G高密度定向包被到超顺磁性微球表面,与当前国际免疫磁珠市场上同类产品相比,该产品具有更多的抗体结合位点,磁珠使用量更少,非特异性结合率低,可便捷高效地进行免疫沉淀实验。每毫升免疫沉淀磁珠结合Human IgG的能力可达到300 μg...
Protein A/G magnetic beads (Thermo Fisher) were added into the antibody-antigen mixture followed by incubation for 1.5 h at 4 °C on a rotator. A magnetic stand was used to collect the beads, followed by washing in lysis buffer four times. The captured complex was eluted with 1X ...
5、 更加容易实现分批上样,从而可使用更小体积的Protein A亲和柱。 WorkBeads™ affimAb专为超纯mAb纯化而设计 单抗药物经常需要重复的高剂量给药,且药物相关的杂质与剂量相关,即使是微量杂质也可能导致很大的累积效应,因此单抗药物治疗对纯度要求非常严格。Bio-Works品牌其琼脂糖纯化树脂WorkBeads™不同于以往的产...