Proteasome Complex Attachment of the ubiquitin peptide to proteins targets them for proteolytic degradation by a complex cellular structure, the proteasome. The regulated proteolysis of proteins by proteasomes removes denatured, damaged or improperly translated proteins from cells and regulates the level of...
Proteasome Complexdoi:10.1007/978-3-662-46875-3_101896Springer, Berlin, Heidelberg
Counter to expectation, reducing expression of individual subunits of the proteasome's 19S regulatory complex increased survival. Strong 19S reduction was cytotoxic but modest reduction protected cells from inhibitors. Protection was accompanied by an increased ratio of 20S to 26S proteasomes, ...
AWestern blot analysis of the different fractions separated by OptiPrep velocity gradient centrifugation. Anti-PSMA1 antibody was used to probe the 20S proteasome complex, and anti-PSMD1 was used to identify the 19S particle. As a positive control, antibodies against the EV markers HSP90 and SR...
Here, we utilize single-particle cryo-electron microscopy (cryo-EM) to determine the structure of the Mpa–proteasome complex at the stage of Pup translocation into the Mpa pore. We resolved two distinct conformational states corresponding to different stages during substrate translocation by the hexam...
(Table 1).The core proteasome complex consists of a barrel-shaped structure, which is referred to as the 20S proteasome, which is comprised of 28 α- and β-subunits. The individual α- and β-subunits are arranged in four separate rings, with each ring consisting of either seven α- or...
The 26S proteasome is a 2.5-MDa, ATP-dependent multisubunit proteolytic complex that processively destroys proteins carrying a degradation signal. The prot... P Sledz,P Unverdorben,F Beck,... - 《Proceedings of the National Academy of Sciences of the United States of America》 被引量: 200...
产品介绍MG-132 is a peptide aldehyde and a potent, reversible, and cell-permeable proteasome inhibitor with an IC50 of 100 nM, and effectively blocks the proteolytic activity of the 26S proteasome complex. Important Note: This product as supplied is intended for research use only, not for use...
In the present study, this ALAD-proteasome complex was further characterized in cultured prostate cancer cells and the effects of SAHA treatment on the regulation of ALAD were investigated. ALAD interacts with the 20S proteasomal core, but not the 19S regulatory cap. Some ubiquitinated species were...
Proteasome is a protein degradation complex that plays a major role in maintaining cellular homeostasis. Despite extensive efforts to identify protein substrates that are degraded through ubiquitination, the regulation of proteasome activity itself under