with promoter mutations to harnessing these regulatory components in biotechnological applications.In this chapter, we describe the manipulation of regulatory elements within promoters, with a specific focus on the use of CRISPR technology on enhancers and silencer elements of the Ovalbumin gene promoter....
The promoter (P) and the −5 kb enhancer (RE) of Nanog are indicated in orange. See also Figure S1 and Tables S1 and S2. Next, TINC was performed with both TALEs in three independent experiments. As negative control, empty vector-transfected ESCs were used. The DNA isolated in each ...
To obtain long-term, neuronal-specific expression, we previously constructed chimeric promoters that fused an upstream enhancer from the TH promoter to the NF-H promoter (TH-NFH promoter) or placed a β-globin insulator (INS) upstream of the TH-NFH promoter (INS-TH-NFH promoter) (Zhang et ...
appears to contain at least one enhancer that initiates Abd-B expression in the early embryo, as well as a PRE silencer element that maintains the expression pattern throughout development (9,16–24). Boundary elements of a specific class are proposed to exist between each iab domain to al...