Ku¨hne, "Proline- rich sequence recognition domain (PRD): ligands, function and inhibition," in Protein-Protein Interactions as New Drug Targets, E. Klussmann and J. Scott, Eds., pp. 407-428, Springer, Berlin, Germany, 2008.Freund C, Schmalz HG, Sticht J, Kühne R. Proline-rich ...
An important class of PPIs comprises proline-rich motifs (PRM) that are specifically recognized by PRM-binding domains (PRD). Aromatic side chains of the PRDs define the binding pockets that often recognize individual proline residues, while flanking sequences mediate specificity. Several of these...
it seems unlikely that the deletion of individual p53-mediated genes will produce a loss of tumor suppressor activity.8–10 We used a genetic model in which the proline-rich domain (PRD) of p53 (p53ΔP) was deleted, resulting in an altered transcription function.11 This mutant allowed us ...
hPLSCR2, a homolog of hPLSCR1 that lacks N-terminal proline-rich domain (PRD), did not show scramblase activity. We attribute this absence of scramblase activity of hPLSCR2 to the lack of N-terminal PRD. Hence to investigate the above hypothesis, we added the PRD of hPLSCR1 to ...
TAp63β(ΔPRD) also functioned as a dominant negative to wild-type p63β in a dose-dependent manner. Furthermore, the loss of function seen with deletion of the proline-rich domain was not due to a DNA-binding defect, as TAp63β(ΔPRD) was found to strongly bind endogenous promoters ...
The SH3 domains of syndapin and endophilin bind the PXXP motifs called Site 2 and 3 (Pro-786–Pro-793) at the N-terminal end of the proline-rich domain, whereas the amphiphysin SH3 binds Site 9 (Pro-833–Pro-836) toward the C-terminal end. In some proteins, SH3/peptide ...
MBD: microtubule-binding domain; PRD: proline-rich domain; Bar: BIN1-amphiphysin-Rvs167; CLAP: clathrin and AP-2 binding; MycBD: Myc-binding domain; SH3: Src homology 3. b. GST pull-down assays performed with various Tau constructs corresponding to specific Tau subdomains (Lane 2: GST-...
These activators were previously shown to bind to a proline/arginine-rich domain (PRD) in the carboxyl-terminal region of the enzyme. Dynamin II, which is ubiquitously expressed, had not been purified or characterized previously. In this study, the enzymatic properties of rat dynamin II and of...
whether it be the central proline-rich domain, or the alpha-helix located in the C-terminus. The identification of the active portion will also perhaps allow to further explain the molecular mechanism by which it can disrupt the bacterial membrane, and be compared to established scenarios put ...
67.5 ?72.5 142.5 162.3 PPP ?70 158.4 Table2:Theproline-richmotif(PRM)andtheproline-richmotifbinding(PRB)domaininimmune-relatedproteins. Proteinreceptor CD28 PRM MYPPPY MYPPPY FDPPP LPPP PRMinteractant CD80 PRDcriticalresidue Evidence Y71 Mutagenesis Membraneassociated CD152 ICOS CD86 F ...