human livers.Organoids as an in vitro system hold the promise to generate organ-like tissues in a dish.Recent advances in human liver organoids also facilitate the understanding of the biology and diseases in this complex organ.Here we review the progress in human liver organoids,mainly ...
Here we review the progress in human liver organoids, mainly focusing on the methods to generate liver organoids, their applications, and possible future directions. 展开 关键词:liver organoid progress perspectives DOI: 10.1093/jmcb/mjaa013 年份: 2020 ...
et al. Vascularized and functional human liver from an iPSC-derived organ bud transplant. Nature 499, 481–484 (2013). This work reports the formation of vascularized hPSC-derived liver organoids, demonstrating the possibility to obtain vascularized organoids by co-culturing stem cells and ...
2D planar cells were cultured in PDMS chambers in majority organ-on-a-chips. More and more studies focused on 3D cells or organoids instead recently due to their better biomimetic capability for drug screening and disease models. In 2018, Qin et al. reported a liver organoid from human iPS...
Self-assembled liver organoids recapitulate hepatobiliary organogenesis in vitro. Hepatology. 2018;67(2):750–61. Article CAS PubMed Google Scholar Kanninen LK, et al. Hepatic differentiation of human pluripotent stem cells on human liver progenitor HepaRG-derived acellular matrix. Exp Cell Res. ...
cells could also be exploited. All of these strategies will help to facilitate the application of stem cells in human medicine. This article reviews the most recent strategies to generate a high amount of mature hepatocyte-like cells and updates current knowledge on liver regenerative medicine....
Generation of C-to-G transversion in mouse embryos via CG editors. Transgenic Res , 2022 , 31: 445 -455 CrossRef Google Scholar [24] Chen Y, Liu J, Zhi S, et al. Repurposing type I-F CRISPR-Cas system as a transcriptional activation tool in human cells. Nat Commun , 2020...
in both form and function.14Although these organoids cannot be considered as true human organs, they have highly similar structures and functions to real human organs and can be stably passaged in vitro. However, it is important to acknowledge that while organoids represent a significant ...
described the existence of an alternative reprogramming route in murine neuronal reprogramming using single-cell RNA-seq. Article PubMed PubMed Central CAS Google Scholar Richards, D. J. et al. Human cardiac organoids for the modelling of myocardial infarction and drug cardiotoxicity. Nat. Biomed...
A Sarkar,MC Marchetto,FH Gage - 《Patterning & Cell Type Specification in the Developing Cns & Pns》 被引量: 0发表: 2020年 Definitive hematopoietic stem/progenitor cells from human embryonic stem cells through serum/feeder-free organoid-induced differentiation Background Ex vivo production of hemato...