The establishment and development of gene knockout mice have provided powerful support for the study of gene function and the treatment of human diseases. Gene targeting and gene trap are two techniques for generating gene knockout mice from embryonic st
The Sanger Institute mouse genetics programme: progress on the high throughput characterisation of knockout micePurpose Anna-Karin Gerdin The Wellcome Trust Mouse Genetics Programme Group Wellcome Trust Sanger Institute, Genome Campus, Hinxton, Cambridgeshire, CB10 1SA, England, UK. Email: mouse...
A. Improving human hemato-lymphoid-system mice by cytokine knock-in gene replacement. Trends Immunol. 32, 321–327 (2011). Article CAS PubMed Google Scholar Ito, M. et al. NOD/SCID/γcnull mouse: an excellent recipient mouse model for engraftment of human cells. Blood 100, 3175–3182 ...
In 2018, Perez-Garcia et al. [4] reported that among 103 gene knockout mice they investigated, almost 70% of embryonic-lethal mice showed disordered placental development. This study strongly emphasized the importance of placentation in supporting embryonic development and brought placental biology ...
KOMP2 collaborates with the International Mouse Phenotyping Consortium (IMPC) to knockout and characterize all protein-coding genes in the mouse genome (https://commonfund.nih.gov/KOMP2/). As creating mice with gene knockouts is a time-consuming and difficult process, the KOMP2 program has ...
This downregulation may be a protective, but ineffectual, response in order to counteract the influx of water and resultant cerebral swelling. Furthermore, unlike in models of vasogenic edema, AQP4 knockout mice exhibited reduced infarct sizes, decreased brain water content, a...
and RNAP II) to stimulate the initiation and extension of transcription.fCRISPR-mediated gene editing mainly uses Cas9 and sgRNA to introduce DSBs at specific positions in the genome effectively. DSBs are generally repaired by HDR or NHEJ, achieving insertion, knockout, and site-specific mutagenesi...
PARP inhibition by olaparib or gene knockout blocks asthma-like manifestation in mice by modulating CD4(+) T cell function. J Transl Med. 2015;13:225.DOI: https://doi.org/10.1186/s12967-015-0583-0. 77. Rosado MM, Bennici E, Novelli F, Pioli C. Beyond ...
We will also perform on KAT6A knockout mice to further confirm the function of KAT6A in bone remodeling and OTM. Fig. 7 Schematic illustration of the KAT6A/YAP/TEAD4 pathway modulating osteoclastogenesis during OTM by regulating the RANKL/OPG ratio Full size image...
In the dextran sulphate sodium (DSS) model, for instance, C3H/HeJBir mice are highly susceptible and dis- play spontaneous colitis, while the C57/black 6 strain is re- sistant (125). Similar findings apply in gene-targeted models of IBD, in addition to chemically induced colitis and are ...