EP0255320A2 * Jul 27, 1987 Feb 3, 1988 Genzyme Corporation Production of proteins in myeloma cellsEP0255320A2 * Jul 27, 1987 Feb 3, 1988 Genzyme Corporation Production de protéines dans des cellules de myélomesEP0255320A2 Jul 27, 1987 Feb 3, 1988 Genzyme Corporation Production of proteins...
The present invention provides methods for the production of proteins, particularly toxic proteins, in host cells. The invention provides methods which use a fusion protein comprising a chaperonin binding domain in host cells induced or regulated to have increased levels of chaperonin which binds the ...
Optimizing the production of recombinant proteins is a problem of major industrial and pharmaceutical importance. Secretion of the protein by the host cell considerably simplifies downstream purification processes. However, for many proteins, this is als
The temperature inducible expression system, based on the pL and/or pR phage lambda promoters regulated by the thermolabile cI857 repressor has been widely use to produce recombinant proteins in prokariotic cells. In this expression system, induction of heterologous protein is achieved by increasing ...
Production of recombinant proteins in insect cells with baculovirus has a long history dating back to the mid 1980s9,10,11. Substantial optimization regarding handling, protein yield, deletion of proteases and other factors was achieved over time12,13,14. Yet, the baculovirus expression vector syste...
Importantly, for more than half of the targets tested the yields obtained using un-induced BL21(DE3) cells were higher than the yields obtained in the widely used membrane protein production strains C41(DE3) and C43(DE3). Since most secretory proteins reach the periplasm via the Sec-translocon...
coli BL21(DE3) cells. These engineered E. coli strains were cultured in shake flasks or in a 3-L fermentor to produce the test proteins. The shake-flask fermentation was performed as follows. A 10-mL aliquot of Luria–Bertani medium (g/L: tryptone, 10.0; yeast extract, 5.0; NaCl, ...
WO1991002066A11991-02-21PRODUCTION OF HETEROLOGOUS PROTEINS IN PLANTS AND PLANT CELLS Other References: Chen et al (1982) Theor. Appl. Genet. 62(2) pp. 103-108. Larkins et al (1985) J. Cell Biochem Suppl. 0 (9 part C). Kush et al (1990) Proc. Natl. Acad Sci USA 87:1787-1790...
Silk-elastin-like proteins (SELPs) combining the physicochemical and biological properties of silk and elastin have a high potential for use in the pharmaceutical, regenerative medicine and materials fields. Their development for use is however restraine
usingcircular dichroism spectroscopyrevealed the proteins to consist of mostly α-helices, as expected. All three UCPs were able to bind GDP, a well-known physiological inhibitor, as shown by theFluorescence Resonance Energy Transfer(FRET) technique, suggesting that the proteins are in a natively ...