Improved N-terminal processing of recombinant proteins synthesized in E. coli. Bio/Technology 13:504–506 - Sandman, Grayling, et al. - 1995 () Citation Context ...idue is present (data not shown). It is likely, therefore, that there is some heterogeneity at the N terminus, as has ...
The search for inexpensive production systems capable of producing large quantities of recombinant protein has resulted in the development of new technology platforms based on transgenic plants and animals. Over the past decade, these transgenic systems have been used to produce several products and pote...
Continuous processing of recombinant proteins: Integration of inclusion body solubilization and refolding using simulated moving bed size exclusion chromat... An integrated process which combines continuous inclusion body dissolution with NaOH and continuous matrix-assisted refolding based on closed-loop simulat...
The analysis of the Dicer cleavage products generated in vitro revealed the presence of a cleavage intermediate when pre-miRNA was processed by recombinant Dicer alone. This intermediate was not observed during pre-miRNA cleavage by endogenous Dicer. We demonstrate that AGO2, PACT and TRBP were ...
Western Blotting of Recombinant Proteins The transfected COS cells were harvested by centrifugation and solubilized with SDS sample buffer in the presence of 5% β-mercaptoethanol. Approximately 2% of the lysate from one 100-mm dish was loaded on an SDS gel. To detect soluble p120, 1 ml of con...
Discusses research which discovered a mechanism of growth-factor activation in line with the functions of transmembrane proteins, Star and Rhomboid-1. Reference to studies published in "Cell" journal; Expression of recombinant Star and Rhomboid-1 in Drosophila; Information on the process known as ...
proteolytically activate such substrates./p pResults/p pIn this study we have aimed at providing an improved assembly of Kex2 target proteins to explain the phenotypes observed in fungal itkex2 /itdeletion mutants by itin vitro /itdigestion of recombinant substrates from itCandida albicans /itand ...
The cystatin superfamily also includes cystatin-like proteins that have no cysteine protease inhibitory activity. A recombinant MCD-1 protein expressed as a GST-fusion protein in Escherichia coli failed to inhibit papain in vitro suggesting that the T. spiralis protein is a new member of the non-...
Effective strategies for host cell protein clearance in downstream processing of monoclonal antibodies and Fc-fusion proteins 来自 Semantic Scholar 喜欢 0 阅读量: 443 作者: Li,Yifeng 摘要: Recombinant therapeutic proteins are typically produced through cell culture process. Host cell proteins (HCPs) ...
Production of recombinant p97 protein For the NMR experiments, full-length human p97 (UniProt P55072) and p97-ND1L (residues 1–480) were produced with N-terminal His6-tag and tobacco etch virus (TEV) cleavage site as previously described11,30. Point mutations were introduced using site-di...