The process of converting epithelial cells into mesenchymal cells is called epithelial-mesenchymal transition (EMT), which prompts non-glandular colonies with mesenchymal features to metastasize more easily, especially when exposed to transforming growth factor beta (TGFβ) [3]. Previous studies indicated...
The present invention describes methods of removing DNA from an RNA transcript during the mRNA production process. The method embodies procedures for obtaining an in vitro transcription product, and removing any DNA from the product. The DNA can be removed by adding either free DNase or a resin ...
Learn about mRNA transcription. Discover where and how DNA is transcribed into RNA in prokaryotes and eukaryotes, and examine the final product of...
Describe the process of translation and transcription in the cell. Central dogma: The central dogma in molecular biology is a theory that states that genetic information flows in only one direction: from DNA (deoxyribose nucleic acid) to RNA and from RNA to proteins. Francis Crick firstly ...
To perform this, it was necessary to transcribe mRNA to DNA, which was used as strands of DNA. Previously DNA was removed by DNAase (included in the purification kit). The mRNAs’ DNA, 1 μL of Primers (hexamers) 0.5 μg/μL, 1 μL of 10 mM dNTPs and water were added to a ...
Transcription factors interact with double-stranded DNA during regulation of transcription. Oligonucleotides can serve as competitive inhibitors of transcription factors to modulate their action. Several recent reports describe such interactions (see Bielinska, A., et. al., Science, 1990, 250, 997-1000...
In contrast, anti-inflammatory features of efferocytic tumour-associated macrophages may be detrimental to an antitumour response, and in this context, converting efferocytosis to a more inflammatory nature could potentially be beneficial. Numerous strategies to therapeutically harness efferocytosis ...
Enzymes that are useful in this embodiment of the invention include, but are not limited to, alkaline phosphatase useful for converting phosphate-containing prodrugs into free drugs; arylsulfatase useful for converting sulfate-containing prodrugs into free drugs; proteases, such as serratia protease, the...
TUMOR NECROSIS FACTOR-a CONVERTASE (ADAM17) MEDIATES REGULATED ECTODOMAIN SHEDDING OF THE SARS- CoV RECEPTOR, ANGIOTENSIN-CONVERTING ENZYME-2 (ACE2) into the Xba I and Xho I sites of the pCI-Neo expression vector (Promega). The sequence fidelity of the insert was confirmed by automated sequenc...
A protein or a polypeptide having an effect of converting 1-propenylsulfenic acid into a lachrymator which participates in the synthesis of the lachrymator released in grinding or cutting a plant such as onion; a DNA encoding the above protein or polypeptide (a lachrymator synthase gene); a ...