报告基因:携带海肾荧光素酶(Renilla luciferase)基因,编码分子量为36 kDa的海肾荧光素酶,翻译后无需修饰。 抗性标记:氨苄青霉素(Ampicillin)抗性。 载体大小:约4045 bp。 克隆位点:具有多克隆位点,方便插入感兴趣的5'-UTR、启动子、增强子等调控元件。
备注:pRL-TK是海肾荧光素酶报告载体,含有SV40增强子 质粒特性 启动子:HSV-TK(单纯疱疹病毒胸腺嘧啶核苷激酶)启动子,提供低至中等水平的组成型表达。 报告基因:携带海肾荧光素酶(Renilla luciferase)基因,编码分子量为36 kDa的海肾荧光素酶,翻译后无需修饰。 抗性标记:氨苄青霉素(Ampicillin)抗性。 载体大小:约4045...
pRL-TK报告基因质粒携带renilla luciferase基因,编码分子量为36kD的海肾萤光素酶,翻译后无需修饰,常用于报告基因检测,本质粒为氨苄抗性。 产品组成: 组分1μg100μg pRL-TK报告基因质粒1μg100μg 说明书1份1份 保存:-20℃ 主要信息: Feature NucleotidePosition ...
moderate levels of Renilla luciferase expression in co-transfected mammalian cells. A Chimeric Intron Downstream of either the constitutive promoter regions (CMV, TK or SV40) or the multiple cloning site of the pRL Vectors is a chimeric intron comprised of ...
Further, we show that for Nurr1, phRG-B (a promoterless reporter plasmid containing synthetic Renilla luciferase gene) is a better control reporter vector than HSV-TK containing vectors. Finally, we noted the lack of effect of Nurr1 protein on the Fas Ligand promoter-driven transcription....
Renilla luciferase is a 36kDa monomeric protein that does not require post- translational modification for activity (2). Therefore, like firefly luciferase, the enzyme may function as a genetic reporter immediately following translation. For information about the use of this plasmid in conjunction ...
In transcription assays, Renilla luciferase-expressing plasmids (more specifically pRL-TK) are commonly used as an internal control of transfection efficiency. Normalization of the experimental reporter gene transcription to the internal control reporter gene transcription minimizes variability of obtained resul...
Renilla-Glo™ Luciferase Assay System Promega E2720ANGPTL7 (human), pAb,产品货号|编号:ALX-210-453-C100,价格及说明书详询!(ENZO:Alexis,Assay designs,Biomol,Stressgen)Omentin 1 (human), pAb,产品货号|编号:ALX-210-454-C100,价格及说明书详询!(ENZO:Alexis,Assay designs,Biomol,Stressgen)ANGPTL3 ...