启动子:HSV-TK(单纯疱疹病毒胸腺嘧啶核苷激酶)启动子,提供低至中等水平的组成型表达。 报告基因:携带海肾荧光素酶(Renilla luciferase)基因,编码分子量为36 kDa的海肾荧光素酶,翻译后无需修饰。 抗性标记:氨苄青霉素(Ampicillin)抗性。 载体大小:约4045 bp。
pRL-TK报告基因质粒带有的HSV TK promoter (单纯疱疹病毒胸腺嘧啶核苷激酶启动子)相对于SV40和CMV增强子/启动子较弱,更适用于共转系统中的内参报告基因。 pRL-TK报告基因质粒携带renilla luciferase基因,编码分子量为36kD的海肾萤光素酶,翻译后无需修饰,常用于报告基因检测,本质粒为氨苄抗性。
simplex virus thymidine kinase (HSV-TK) promoter to provide low to moderate levels of Renilla luciferase expression in co-transfected mammalian cells. A Chimeric Intron Downstream of either the constitutive promoter regions (CMV, TK or SV40) or ...
Further, we show that for Nurr1, phRG-B (a promoterless reporter plasmid containing synthetic Renilla luciferase gene) is a better control reporter vector than HSV-TK containing vectors. Finally, we noted the lack of effect of Nurr1 protein on the Fas Ligand promoter-driven transcription....
pRL-SV40-C(报告基因质粒)是信裕生物自行研发的用于在哺乳动物细胞中进行海肾萤光素酶(Renilla luciferase)报告基因检测的质粒,主要用于在其多克隆位点插入感兴趣的3' -UTR、启动子、增强子等调控元件研究该调控序列的基因转录调控活性,或用作萤火虫荧光素酶(firefly luciferase)报告基因检测时的内参。也可以使用Bgl II...
In transcription assays, Renilla luciferase-expressing plasmids (more specifically pRL-TK) are commonly used as an internal control of transfection efficiency. Normalization of the experimental reporter gene transcription to the internal control reporter gene transcription minimizes variability of obtained resul...
pRL-TK Vector base 1264 (T→C) to eliminate an internal BglII site, base 1807 (T→C) to eliminate internal BamHI site, base 1840 (C→T) to eliminate internal NarI, KasI, BanI and AcyI sites. Description The pRL Reporter Vectors contain a cDNA (Rluc) encoding Renilla luciferase, ...
pRL-SV40-N(报告基因质粒)是沪震生物自行研发的用于在哺乳动物细胞中进行海肾萤光素酶(Renilla luciferase)报告基因检测的质粒,主要用于在其多*位点插入感兴趣的5' -UTR、启动子、增强子等调控元件研究该调控序列的基因转录调控活性,或用作萤火虫荧光素酶(firefly luciferase)报告基因检测时的内参。也可以使用Bgl II和...
By shortened protein half-life we are not talking about the physical presence of the proteins in the cells (i.e. faster degradation of the Renilla luciferase as a result of proteolytic degradation or so-called chemical stability) but rather a shorter functional half-life of the Renilla ...
pRL-SV40 renilla luciferase传染媒介那 相关内容 aBut you’ll still have to learn the new word. You may even have to relearn it many times before you finally actually learn it. 但您更将必须学会新的词。 在您最后实际上学会它之前,您可以甚而必须重学它许多次。[translate] ...