The principle of ligation detection reaction.Jarmo, RitariJenni, HultmanRita, FingerroosJussi, TarkkanenJanne, PullatLars, PaulinNiina, KiviPetri, AuvinenEeva, Auvinen
"tagmentation" combines the fragmentation and ligation reactions into single step that greatly increases the efficiency of the library preparation process. Adapter-ligated fragments are then PCR amplified and gel
The ability of whole cells to catalyse multistep reactions, often yielding synthetically demanding compounds later used by industrial biotech or pharma, makes them an indispensable tool of synthetic chemistry. The complex reaction network employed by cellular catalysts and the still only moderate predictiv...
After adding the adapters, the libraries need to be checked to ensure proper adapter ligation. Gel electrophoresis can be used to check the size and purity of the amplified products and confirm the presence of the target amplicons. DNA Library Preparation 4. Sequencing Once the libraries are prep...
Initially this technique was used for only very small procedures (such as a tubal ligation, “having your tubes tied”), but as surgeons became more proficient, the complexity of the cases that could be performed in this manner increased. The utility of this technique was recognized, and in ...