The principle of ligation detection reaction.Jarmo, RitariJenni, HultmanRita, FingerroosJussi, TarkkanenJanne, PullatLars, PaulinNiina, KiviPetri, AuvinenEeva, Auvinen
Initially this technique was used for only very small procedures (such as a tubal ligation, “having your tubes tied”), but as surgeons became more proficient, the complexity of the cases that could be performed in this manner increased. The utility of this technique was recognized, and in ...
PCR amplification of ARSB exons and adjacent intronic regions, as well as from the promoter region of the gene, was done with specific primers. Each PCR reaction was carried out with specific primers using approximately 40 ng of genomic DNA, 1X the PCR reaction mix ImmoMix™ Red (Bioline...