Understanding gene expression will require understanding where regulatory factors bind genomic DNA. The frequently used sequence-based motifs of protein-DNA binding are not predictive, since a genome contains many more binding sites than are actually bou
Single extractions of genomic DNA of samples from collections B and C used an optimised CTAB method following Chen et al.26. The CTAB buffer consisted of 2% (w/v) CTAB diluted in 100 mM Tris–HCl, 20 mM EDTA, and 1.4 M NaCl; 0.2% (v/v), β-mercaptoethanol was added immediately be...
Here we will provide some example of the TBP application and will discuss its features and uses in comparison with the DNA sequencing-based methods. Keywords: food authentication; metabarcoding; TBP; genotyping 1. Introduction This contribution is inspired by few simple concepts that, applied to ...
Using digital microscopy, scabies was diagnosed in 24 out of 111 participants and confirmed by the extraction of a Sarcoptes mites from the acarine eminence. A characteristic tunnel (burrow) with or without mite could be clearly identified irrespective of the degree of pigmentation of the skin. ...