Development of real-time RT-PCR for evaluation of JEV clearance during purification of HPV type 16 L1 virus-like particles Insect cell culture has greatly increased in part due to the widespread use of insect virus-based vectors for efficient expression of foreign proteins. Ins... HS Jeong,JH...
Production of succinic acid through overexpression of NAD(+)-dependent malic enzyme in an Escherichia coli mutant. NAD(+)-dependent malic enzyme was cloned from the Escherichia coli genome by PCR based on the published partial sequence of the gene. The enzyme was overex... L Stols,MI ...
aTo establish the specificity of the primers used in this PCR method, 39 different bacterial strains, including STEC and non-STEC strains such as Salmonella, were used. All of the STEC strains were positive and all non-STEC organisms were negative. 要建立用于这个PCR方法的底漆的特异性, 39不...
这不可能是作为您的苹果计算机公司ID或是所有密码您在去年使用了的一样。 [translate] aThe primers used for the qRT-PCR analysis, potential gene functions, and amplicon sizes are shown in Table 5. 用于qRT-PCR分析、潜在的基因作用和amplicon大小的底漆在表5显示。 [translate] ...
Primers used in RT-PCR.Kai YaoWei Ge
Primers specific for the genes tfdD, tfdE and tfdF, derived from conserved amino acid sequence motifs of the corresponding homologous enzymes, and primers specific for the genes tfdA and tfdB as well as tfdC taken from the literature were applied in PCR reactions using the genomic DNA of ...
Melting temperature (Tm): The optimal melting temperature of the primers is 60–64°C, with an ideal temperature of 62°C, which is based on typical cycling and reaction conditions and the optimum temperature for PCR enzyme function. Ideally, the melting temperatures of the 2 primers should ...
The prey template then showed strong PCR amplification where none was detectable without the addition of blocking primer. Our method was applied to identifying the winter food of one of the most abundant animals in the world, the Antarctic krill, itEuphausia superba/it. Dietary item DNA was PCR...
Why is an RNA primer needed in a DNA replication? Why are primers needed for DNA replication? A. The primers help with the proofreading function of DNA polymerase. B. They help with the joining of Okazaki fragments. C. A tiny amount of RNA is needed to tell the cell where genes are...
aMal stranded on third 在第三搁浅的Mal[translate] aSuper G Mode 超级G方式[translate] athere was a shower 有阵雨[translate] aGene-special primers used in the real-time quantitative reverse transcription PCR 用于实时定量反向副本的基因特别底漆PCR[translate]...