WATANABE SATOSHI渡辺 聡HIRAO NOBUJI平尾 宜司
插入控制和引物/探针套装,用于DNA分离又称Spike-in Control & Primer/Probe Set For DNA isolation,是由Norgen Biotek品牌生产的高品质产品,相对于同类产品具有极高的性价比。Norgen Biotek成立于2084年,公司总部位于加拿大,是一家著名全球化生物技术公司,生产基于专利技术的核酸和蛋白质纯化及富集产品用于研究和诊断。
probe annealing information. This is the relevant information needed to order your primer/probe set. To print, click and drag, highlighting the information you want and selecting "Copy" from the "Edit" menu, placing it on the clipboard. This should be everything from the Sequence name through...
⑥在Primers/Probes界面中会将每对Primers/Probe的组合列出来,请从中挑选出适当的组合,挑选方式可依据之前的primer/probedesignguideline。在MGBProbe的筛选中,亦在Parameters界面中加选C比G多的序列作为进一步筛选的参数。 ⑦決定primer/probeset之后则可进行存档,存档的方式从File→SaveAs存档。如果想将所选择的...
(2 Probe 列 綠 ) 藍 Forward Primer Reverse Primer (* 不 ) Primer Express Operation Guide ”Primers / Probes ” Tab Primers/Probe 列 來 2 primer/ probe design guideline MGB Probe ”Parameters ” Tab ”C ”” G ” 列 參數 primer/ probe set 行 File → Save As primer/ probe set ...
The Penalty score assigned to your Primer Probe set is based on factors such as amplicon length. Since the default TaqMan? design parameters keep amplicons under 150 bp, this can be disregarded as well. Primer/probe sets will be listed when the search is complete. Scroll to the right to ...
designguidelines.ThePenaltyscoreassignedtoyourPrimer&Probesetisbasedonfactorssuchasampliconlength.SincethedefaultTaqMan?designparameterskeepampliconsunder150bp,thiscanbedisregardedaswell.Primer/probesetswillbelistedwhenthesearchisplete.ScrolltotherighttoviewtheProbes.Clickonthe"Start"headingunderprobesto sort probes...
”Sequence” Tab Probe ? ) ”Primers / Probes” Tab Forward Primer Primers/Probe: Reverse Primer (* ? Primer Express Operation Guide ”Primers / Probes” Tab 2 ”C ””G” Primers/Probe ? ? MGB Probe ”Parameters” Tab primer/ probe design guideline ? ?? primer/ probe set primer/...
TaqMan Probe Primer Probe 与Primer 的距离愈近愈好, PCR产物大小建议在50-150 bp为最佳 G/C % 为30-80 % 避免有重复序列的出现,尤其避免4个以上G的出现 Tm 值: 68-70℃ (Quantification assay) Tm 值: 58-60℃ 65-67℃ (Allelic Discrimination assay)Probe 长: Primer 长: 13~25 bases (TaqMan ...
Probe 度:13~25 bases (TaqMan MGB probe) 13~30 bases (TaqMan probe) Primer 度: 20 bases (Optimal)連 6 A 列 5’ 列不 G ( FAM-dye 5’ 列不 G)C G strand probe b 3’ 4 列裡 3 G (GGG-MGB-3’ or GGAG-MGB-3’) a probe 2 CC di-nucleotides a 3’ 列裡不 2 C+G a: ...