Kimura Y, Soma T, Kasahara N, Delobel D, Hanami T, Tanaka Y, et al. (2016) Edesign: Primer and Enhanced Internal Probe Design Tool for Quantitative PCR Experiments and Genotyping Assays . PLoS ONE 11 ( 2 ): e0146950 doi: 10.1371/journal.pone.0146950...
如果是设计SYBRGreen引物,也要选择TaqMan Primer and Probe design并遵守这些规则,但是只需要合成引物就可以了。 TaqMan探针: 1.保持G-C含量在30-80%之间。 2.避免同一碱基重复过多。特别是G,不可超过4个及以上。 3. 5' end不能是G。 4.尽量使探针中的Cs多于Gs。如果不能满足,则使用互补链上的探针。 5...
primer, Na+, and Mg2+concentrations), force use of a particular primer or probe sequence, and/or specify design regions. Alternatively, if you originally chosePCRor qPCR designs, you may click onCustomize Assay Designfrom yourResultspage to adjust design parameters (Figure 2). This step provide...
如果是设计SYBRGreen引物,也要选择TaqManPrimerandProbedesign并遵守这些规如此,但是只需要合成引物就可以了。TaqMan探针:1.保持G-C含量在30-80%之间。2.防止同一碱基重复过多。特别是G,不可超过4个与以上。3.5'end不能是G。4.尽量使探针中的Cs多于Gs。如果不能满足,如此使用互补链上的探针。5.对于单探针反响,...
Reverse Primer ”Sequence” Tab Reverse 列 highlight 來( 25 bases 度 切 Probe 列 ) 利 Edit →” Copy Complement ” 列 Tools → Primer Probe Test Tool Rev Primer 欄 Reverse Primer Tm 58-60 Tm 不 Rev Primer 不 列 Tm, %GC 度 Primer ( 參 primer/ probe design guideline) 若 ”Trim ...
FastPCR: An in silico tool for fast primer and probe design and advanced sequence analysisDNA fingerprintingDNA primersDegenerate PCR... - 《Genomics》 被引量: 0发表: 2017年 Introduction on Using the FastPCR Software and the Related Java Web Tools for PCR and Oligonucleotide Assembly and Analysis...
FastPCR software for PCR primer and probe design and repeat search FastPCR consists of a data editor, build commands for probe and primer design, and automation tools. The software selects the best primers with the ... R Kalendar,D Lee,AH Schulman - 《Genes》 被引量: 436发表: 2009年 Us...
Primer Express v3.0 Primers and Probe Design For Real-Time PCR Primers/Probes Design Guideline TaqMan Probe Primer Probe Primer 離 , PCR 50-150 bp G/C % 30-80 % 列 4 G Tm : 68-70 (Quantification assay)65-67 (Allelic Discrimination assay)Tm : 58-60 Probe 度:13~25 bases (TaqMan...
06 January 2016 Published: 08 February 2016 PrimerMapper: high throughput primer design and graphical assembly for PCR and SNP detection Damien M. O'Halloran1,2 Primer design represents a widely employed gambit in diverse molecular applications including PCR, sequencing, and probe hybridization. ...
Also, access our easy oligo ordering portal or primer and probe design tools. Featured oligonucleotides, primers, and custom synthesis services Custom DNA oligos Customize your oligos with standard deoxynucleotides, modified bases, and 5′- and 3′-modifications, available in up to five quan...