Keywords: primary cells; cell lines; immortalization; in vitro cell culture; standardizationdoi:10.1038/npg.els.0003960Glyn StaceyJohn Wiley & Sons, LtdStacey G (2006) Primary cell cultures and immortal cell lines. eLS. https:// doi.org/10.1038/npg.els.0003960...
In the earlier and mid decades of the 20th century clinical observation was supported by morphological characterization using EM and by immunofluorescence methods and an increased use of primary tissue culture and eventually immortal cell lines. The development of serological techniques for the detection ...
Infection of human primary B-lymphocytes with Epstein-Barr virus (EBV) drives quiescent cells into continual proliferation and results in the outgrowth of immortal cell lines. This requires re-programming of the mechanisms that, in the absence of appropriate antigenic stimulation, normally prevent the...
“driver”mutations contributing to the development of the cancers in 119 genes. Out of 210 cancers analyzed, 169 were primary tumors, 2 were early cultures, and 39 were immortal cancer cell lines. Pre 多数体壁变化预计是“对oncogenesis不贡献的乘客”,但是证据为“贡献对癌症的发展的司机”变化在...
This immortal strand hypothesis proposed by Cairns in 1975 [40] predicts that the stem cell DNA will not be labeled with pulsed nucleotide analogs after their first division; hence these markers would be ineffective in revealing the 1st-generation neuronal precursors. We believe this situation does...
Cellular prion protein suppressess the apoptotic cell death by mediating the intracellular H 2 O 2 in primary culture and immortalized neuronal cellsTo know the mechanisms in which cellular isoform of prion protein (PrPC) is involved for the neuroprotection, we compared death signals in Prnp-/- ...
CRC is a heterogeneous disease. In the present study, a novel cell line (cc-006cpm8) originating from moderately/poorly differentiated CRC with a Chinese genetic background was established as few have been reported previously. The cc-006cpm8 cells formed an immortal cell line that were passage...
Alpha TC1 (αTC1) and Beta-TC-6 (βTC6) mouse islet cell lines are cellular models of islet (dys)function and type 2 diabetes (T2D). However, genomic characteristics of these cells, and their similarities to primary islet alpha and beta cells, are undefined. Here, we report the epigeno...
Primary CA1 and conditionally immortal MHP36 cell grafts restore conditional discrimination learning and recall in marmosets after excitotoxic lesions of the hip- pocampal CA1 field. Brain 122: 2321-2335.Virley D, Ridley RM, Sinden JD, et al. Primary CA1 and conditionally immortal MHP36 cell...
Pantic B, Borgia D, Giunco S, Malena A, Kiyono T, Salvatori S, et al. Reliable and versatile immortal muscle cell models from healthy and myotonic dys- trophy type 1 primary human myoblasts. Exp Cell Res (2016) 342(1):39-51. doi:10.1016/j.yexcr.2016.02.013...