Suh JKF, Hyung S (2018) Primary motor neuron culture to promote cellular viability and myelination. In Neurotrophic Factors. Methods Mol Biol 1727: 403-411.Suh, J. F., and Hyung, S. (2018). Primary motor neuron culture to promote cellular viability and myelination. Methods Mol. Biol. ...
Motor Neuron (MN) Culture. MNs were harvested from the spinal cord of CD-1 mice (E14 fetus) as previously described24 with some modifications. Ventral horns of the spinal cord (L1-L6 segment) were each collected in HBSS and 1% TLR trypsin (Worthington) was added for 15 min in a ...
Cell types and disease models cited in publications using Gibco neuronal cell culture reagents Primary neurons Brainstem, cerebellar, cortical, dopaminergic, DRG, enteric, GABAergic, glutamatergic, hippocampal, motor, neural progenitor, pancreatic, parasympathetic, retinal, septal, striatal, substantia...
We have designed a cell culture system for thoracic neurons of adult Locusta migratoria that enables the establishment of functional synapses in vitro. Patch-clamp recordings revealed three different neuron classes. About half of the neurons (47%) had unexcitable somata with outward and no inward ...
We combined the use of Drosophila models for FUS proteinopathies with that of the primary neuronal cultures to address neuron-to-neuron transfer of FUS proteins. Using conditional co-culture models and an optimized flow cytometry-based methodology, we demonstrated that ALS-mutant forms of FUS ...
Charcot-Marie-Tooth DiseaseIn Vitro ModelingPrimary Motor NeuronsTransfectionNeurobiologyMotor Neuron PolarityAxon GuidanceAxon TraffickingNeurodegenerative MechanismsEnriched Motor Neuron CultureKnockdown ProteinSpinal Cord DissectionEnzymatic DigestionCell SuspensionDensity Gradient CentrifugationMotoneuron Enrichmen...
Synthetic biodegradable polymers including poly(lactic acid) (PLA) are attractive cell culture substrates because their surfaces can be micropatterned to support cell adhesion. The cell adhesion properties of a scaffold mainly depend on its surface chemical and structural features; however, it remains un...
A limitation of our work is that the neuron culture only represents one of the many cells located in the central nervous system that models a single-direction pathway where neurons respond to different disease-associated metabolites. While it is important to note that astrocytes and microglia are ...
Amyotrophic Lateral Sclerosis is the most prominent of the five motor neuron diseases, distinguishing itself from the others through degeneration of both upper motor neurons (UMN) and lower motor neurons (LMN) respectively. The "Father of neurology" Jean-Marie Charcot founded the disease in 1869 ...
To specifically assess the role of CXCR2 in the neurotoxicity induced by MIP-2, we used the CXCR1/2 inhibitor reparixin and studied the effect of the chemokine on motor neuron cultures from CXCR2-deficient mice. Methods: Primary motor neurons prepared from rat or mouse embryos were treated ...