Primary cultures of motor neurons, either purified by immunopanning or co-cultured with glial cells and other neuronal cells were used to evaluate the effect of EPO after serum/BDNF deprivation or upon exposure of motor neuron cultures for 48 h to equipotent concentration of two glutamate agonists...
We are particularly interested in neurodegenerative diseases and so altering cell death for the purposes of cell culture would defeat the object of the investigations. This chapter describes our principal sensory and motor neuron cell culture techniques....
Cell types and disease models cited in publications using Gibco neuronal cell culture reagents Primary neurons Brainstem, cerebellar, cortical, dopaminergic, DRG, enteric, GABAergic, glutamatergic, hippocampal, motor, neural progenitor, pancreatic, parasympathetic, retinal, septal, striatal, substanti...
We combined the use of Drosophila models for FUS proteinopathies with that of the primary neuronal cultures to address neuron-to-neuron transfer of FUS proteins. Using conditional co-culture models and an optimized flow cytometry-based methodology, we demonstrated that ALS-mutant forms of FUS ...
To specifically assess the role of CXCR2 in the neurotoxicity induced by MIP-2, we used the CXCR1/2 inhibitor reparixin and studied the effect of the chemokine on motor neuron cultures from CXCR2-deficient mice. Methods: Primary motor neurons prepared from rat or mouse embryos were treated ...
Synthetic biodegradable polymers including poly(lactic acid) (PLA) are attractive cell culture substrates because their surfaces can be micropatterned to support cell adhesion. The cell adhesion properties of a scaffold mainly depend on its surface chemical and structural features; however, it remains un...
Primary neuron culture Postnatal day 0–2 (P0–P2) mouse pups were sacrificed by decapitation. Cerebral cortices were dissected and minced prior to incubation in enzyme digestion solution (1 × hibernate complete medium, 0.06 mg/mLl-cysteine (Sigma, 778672), 1.4 × 10−2N NaOH (Sigm...
central nervous system (CNS) using both an in vivo rat model, and in vitro culture systems of human primary astrocytes and a microglia cell line, HMO... Abdullah,Md.,Sheikh,... - 《Glia》 被引量: 40发表: 2009年 Estrogen, neuroinflammation and neuroprotection in Parkinson's disease : key...
Neuron cell culture mediumThermoFisher ScientificA3582901Neurobasal Plus medium HBSSSigma-AldrichH6648-500ML HEPES buffer 1 MThermoFisher Scientific15630056 Density gradiant mediumSigma-AldrichD1556Optiprep supplement mediumThermoFisher ScientificA3582801B-27 Plus ...
iPSC differentiation into proprioceptor-enriched primary sensory neuron cultures Figure 1 shows a summary of the steps of the differentiation protocol. The process of iPSC differentiation in culture recapitulated the in vivo DRG development. Initially, iPSCs proliferated in a uniform confluent layer, wit...